Ontology highlight
ABSTRACT:
SUBMITTER: Mamontova AV
PROVIDER: S-EPMC6125319 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Mamontova Anastasia V AV Solovyev Ilya D ID Savitsky Alexander P AP Shakhov Alexander М AМ Lukyanov Konstantin A KA Bogdanov Alexey M AM
Scientific reports 20180905 1
Fluorescence lifetime imaging microscopy (FLIM) measures fluorescence decay rate at every pixel of an image. FLIM can separate probes of the same color but different fluorescence lifetimes (FL), thus it is a promising approach for multiparameter imaging. However, available GFP-like fluorescent proteins (FP) possess a narrow range of FLs (commonly, 2.3-3.5 ns) which limits their applicability for multiparameter FLIM. Here we report a new FP probe showing both subnanosecond fluorescence lifetime a ...[more]