Dataset Information

Proceeding From in vivo Functions of Pheromone Receptors: Peripheral-Coding Perception of Pheromones From Three Closely Related Species, Helicoverpa armigera, H. assulta, and Heliothis virescens.

ABSTRACT: Three closely related species, Helicoverpa armigera, H. assulta, and Heliothis virescens from Lepidoptera Noctuidae, are used as a model system for exploring sexual communication and species isolation. Pheromone receptors (PRs) previously discovered in model moth species include seven in H. armigera, six in H. assulta, and six in H. virescens. PRs named OR6, OR13, and OR16 among these species were found to be functional, characterized by an in vitro Xenopus oocytes system. Using an in vivo transgenic fly system, functional assays of OR6, OR13, and OR16 clades from three closely related Noctuidae species showed that OR13 function was highly conserved, whereas OR6 and OR16 exhibited functional divergence. Similar results were produced from assays in the Xenopus oocytes system. Combined with earlier behavioral results and electrophysiological recordings, we found corresponding relationships among pheromones, PRs, and neurons at the periphery sensory system of each species. Our results provide vital information at the neuronal and molecular level, shedding insight into the sexual communication of closely related species in Lepidoptera.

SUBMITTER: Wang B

PROVIDER: S-EPMC6125646 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Proceeding From in vivo Functions of Pheromone Receptors: Peripheral-Coding Perception of Pheromones From Three Closely Related Species, Helicoverpa armigera, H. assulta, and Heliothis virescens.

Wang Bing B Liu Yang Y Wang Gui-Rong GR

Frontiers in physiology 20180830

Three closely related species, Helicoverpa armigera, H. assulta, and Heliothis virescens from Lepidoptera Noctuidae, are used as a model system for exploring sexual communication and species isolation. Pheromone receptors (PRs) previously discovered in model moth species include seven in H. armigera, six in H. assulta, and six in H. virescens. PRs named OR6, OR13, and OR16 among these species were found to be functional, characterized by an in vitro X ...[more]

PMID: 30214413

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Project description:BackgroundThe chemical components of sex pheromones have been determined for more than a thousand moth species, but so far only a handful of genes encoding enzymes responsible for the biosynthesis of these compounds have been identified. For understanding the evolution of moth sexual communication, it is essential to know which genes are involved in the production of specific pheromone components and what controls the variation in their relative frequencies in the pheromone blend. We used a transcriptomic approach to characterize the pheromone gland of the Noctuid moth Heliothis virescens, an important agricultural pest, in order to obtain substantial general sequence information and to identify a range of candidate genes involved in the pheromone biosynthetic pathway.ResultsTo facilitate identifying sets of genes involved in a broad range of processes and to capture rare transcripts, we developed our majority of ESTs from a normalized cDNA library of Heliothis virescens pheromone glands (PG). Combining these with a non-normalized library yielded a total of 17,233 ESTs, which assembled into 2,082 contigs and 6,228 singletons. Using BLAST searches of the NR and Swissprot databases we were able to identify a large number of putative unique gene elements (unigenes), which we compared to those derived from previous transcriptomic surveys of the larval stage of Heliothis virescens. The distribution of unigenes among GO Biological Process functional groups shows an overall similarity between PG and larval transcriptomes, but with distinct enrichment of specific pathways in the PG. In addition, we identified a large number of candidate genes in the pheromone biosynthetic pathways.ConclusionThese data constitute one of the first large-scale EST-projects for Noctuidae, a much-needed resource for exploring these pest species. Our analysis shows a surprisingly complex transcriptome and we identified a large number of potential pheromone biosynthetic pathway and immune-related genes that can be applied to population and systematic studies of Heliothis virescens and other Noctuidae.

Project description:Helicoverpa assulta is a pest that causes severe damage to tobacco, pepper and other cash crops. A local strain of HearNPV-BJ (formerly Helicoverpa assulta nucleopolyhedrovirus (HeasNPV-DJ0031)) was isolated from infected H. assulta larvae in Beijing, which had been regarded as a new kind of baculovirus in previous studies. Describing the biological characteristics of the strain, including its external morphology, internal structure and the pathological characteristics of the infection of various cell lines, can provide references for the identification and function of the virus. HearNPV-BJ virion was defined as a single-nucleocapsid nucleopolyhedrovirus by scanning electron microscopy. QB-Ha-E-5 (H. armigera) and BCIRL-Hz-AM1 (H. zea) cell lines were sensitive to HearNPV-BJ. Undoubtedly modern developed sequencing technology further facilitates the increasing understanding of various strains. The whole genome sequence of the HearNPV-BJ was sequenced and analyzed. The HearNPV-BJ isolate genome was 129, 800 bp nucleotides in length with a G + C content of 38.87% and contained 128 open reading frames (ORFs) encoding predicted proteins of 50 or over 50 amino acids, 67 ORFs in the forward orientation and 61 ORFs in the reverse orientation, respectively. The genome shared 99% sequence identity with Helicoverpa armigera nucleopolyhedrovirus C1 strain (HearNPV-C1), and 103 ORFs had very high homology with published HearNPV sequences. Two bro genes and three hrs were found to be dispersed along the HearNPV-BJ genome. Three of the highest homologs, ORFs with HearNPV, were smaller due to the earlier appearance of the stop codon with unknown functions. P6.9 of HearNPV-BJ, a structural protein, is distinctly different from that of Autographa californica nucleopolyhedrovirus (AcMNPV); its homology with the corresponding gene in HearNPV-C1 was 93.58%. HearNPV-BJ contains 38 core genes identified in other baculoviruses, and phylogenetic analysis indicates HearNPV-BJ belongs to Alphabaculovirus Group II, same as HearNPV-C1. The resulting data provide a better understanding of virion structure, gene function and character of infection. By supplementing the whole-genome sequencing data and Kimura-2 model index, there is more evidence to indicate that HearNPV-BJ may be a variant of Helicoverpa armigera nucleopolyhedrovirus, which also deepens our understanding of the virus species demarcation criteria.

Dataset Information

Proceeding From in vivo Functions of Pheromone Receptors: Peripheral-Coding Perception of Pheromones From Three Closely Related Species, Helicoverpa armigera, H. assulta, and Heliothis virescens.

Publications

Proceeding From <i>in vivo</i> Functions of Pheromone Receptors: Peripheral-Coding Perception of Pheromones From Three Closely Related Species, <i>Helicoverpa armigera, H. assulta</i>, and <i>Heliothis virescens</i>.

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