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Organic Extract of Justicia pectoralis Jacq. Leaf Inhibits Interferon-? Secretion and Has Bacteriostatic Activity against Acinetobacter baumannii and Klebsiella pneumoniae.


ABSTRACT: Justicia pectoralis Jacq. (Acanthaceae) leaves currently found in the Brazilian north-east are widely used to treat diabetes, menstrual pains, asthma, and other disorders. This work aimed to identify the phytochemical characterization and biological activities of J. pectoralis leaf extracts. The plant material was ground and the crude extracts were obtained with water or acetone: water (7:3 v/v), yielding aqueous (JPA), and organic (JPO) extracts. Phytochemical characterization was performed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). Cytotoxicity was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay and trypan blue (TB) exclusion assay in peripheral blood mononuclear cells (PBMCs), BALB/c splenocytes, and neoplastic cells (TOLEDO, K562, DU-145, and PANC-1) at 1, 10, and 100??g/mL. Antibacterial activity was evaluated using the microdilution test to obtain the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Cytokines, IFN-?, and IL-17A from culture supernatants of BALB/c mice splenocytes were measured by sandwich ELISA. In the TLC analysis, both JPA and JPO extracts presented coumarin and flavonoids. In addition, HPLC was able to identify coumarin, apigenin, and ellagic acid in both extracts. JPO IC50 was 57.59 ± 1.03??g/mL (MTT) and 69.44 ± 8.08??g/mL (TB) in TOLEDO. MIC value of JPO against Acinetobacter baumannii and Klebsiella pneumoniae was 500??g/mL. JPO (100??g/mL) significantly inhibited IFN-? levels (p=0.03). J. pectoralis is a potential candidate to be further investigated as an IFN-? inhibitory agent and against Acinetobacter baumannii and Klebsiella pneumoniae.

SUBMITTER: Nunes TRS 

PROVIDER: S-EPMC6126107 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Organic Extract of <i>Justicia pectoralis</i> Jacq. Leaf Inhibits Interferon-γ Secretion and Has Bacteriostatic Activity against <i>Acinetobacter baumannii</i> and <i>Klebsiella pneumoniae</i>.

Nunes Tiago Rafael de Sousa TRS   Cordeiro Marina Ferraz MF   Beserra Fernanda Gomes FG   de Souza Matheus Landim ML   da Silva Wliana Alves Viturino WAV   Ferreira Magda Rhayanny Assunção MRA   Soares Luiz Alberto Lira LAL   Costa-Junior Sérgio Dias SD   Cavalcanti Isabella Macário Ferro IMF   Pitta Maira Galdino da Rocha MGDR   Pitta Ivan da Rocha IDR   Rêgo Moacyr Jesus Barreto de Melo MJBM  

Evidence-based complementary and alternative medicine : eCAM 20180823


<i>Justicia pectoralis</i> Jacq. (Acanthaceae) leaves currently found in the Brazilian north-east are widely used to treat diabetes, menstrual pains, asthma, and other disorders. This work aimed to identify the phytochemical characterization and biological activities of <i>J. pectoralis</i> leaf extracts. The plant material was ground and the crude extracts were obtained with water or acetone: water (7:3 v/v), yielding aqueous (JPA), and organic (JPO) extracts. Phytochemical characterization was  ...[more]

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