Ontology highlight
ABSTRACT:
SUBMITTER: Jenkins KA
PROVIDER: S-EPMC6126725 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Jenkins Kelly A KA Fossat Martin J MJ Zhang Siwen S Rai Durgesh K DK Klein Sean S Gillilan Richard R White Zackary Z Gerlich Grayson G McCallum Scott A SA Winter Roland R Gruner Sol M SM Barrick Doug D Royer Catherine A CA
Proceedings of the National Academy of Sciences of the United States of America 20180813 35
The effect of introducing internal cavities on protein native structure and global stability has been well documented, but the consequences of these packing defects on folding free-energy landscapes have received less attention. We investigated the effects of cavity creation on the folding landscape of the leucine-rich repeat protein pp32 by high-pressure (HP) and urea-dependent NMR and high-pressure small-angle X-ray scattering (HPSAXS). Despite a modest global energetic perturbation, cavity cr ...[more]