Intrinsic refractive index matched 3D dSTORM with two objectives: Comparison of detection techniques.
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ABSTRACT: We have built a setup for 3D single molecule localisation microscopy (SMLM) where a very high resolution is achieved by, firstly, the use of two objectives instead of one and, secondly, minimizing optical aberrations by refractive index matching with a glycerol-water mixture as immersion medium in conjunction with glycerol-immersion objectives. Multiple optical paths of the microscope allow to switch between astigmatic and interferometric localisation along the optical axis, thus enabling a direct comparison of the performance of these localisation methods.
SUBMITTER: Schmidt NC
PROVIDER: S-EPMC6127109 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
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