Effects of knockout of the receptor for advanced glycation end-products on bone mineral density and synovitis in mice with intra-articular fractures.

ABSTRACT: Our group employed the mouse closed intra-articular fracture (IAF) model to test the hypothesis that the innate immune system plays a role in initiating synovitis and post-traumatic osteoarthritis (PTOA) in fractured joints. A transgenic strategy featuring knockout of the receptor for advanced glycation end-products (RAGE -/- ) was pursued. The 42 and 84?mJ impacts used to create fractures were in the range previously reported to cause PTOA at 60 days post-fracture. MicroCT (?CT) was used to assess fracture patterns and epiphyseal and metaphyseal bone loss at 30 and 60 days post-fracture. Cartilage degeneration, synovitis, and matrix metalloproteinase (MMP-3, -13) expression were evaluated by histologic analyses. In wild-type mice, ?CT imaging showed that 84?mJ impacts led to significant bone loss at 30 days (p?

SUBMITTER: Seol D 

PROVIDER: S-EPMC6128287 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature