Ontology highlight
ABSTRACT:
SUBMITTER: Joseph A
PROVIDER: S-EPMC6130422 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Acta crystallographica. Section F, Structural biology communications 20180829 Pt 9
The transcriptional activity of RNA polymerase (RNAP) is controlled by a diverse set of regulatory factors. A subset of these regulators modulate the activity of RNAP through its secondary channel. Gre factors reactivate stalled elongation complexes by enhancing the intrinsic cleavage activity of RNAP. In the present study, the protein MSMEG_6292, a Gre-factor homologue from Mycobacterium smegmatis, was expressed heterologously in Escherichia coli and purified using standard chromatographic tech ...[more]