Dataset Information

Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea.

ABSTRACT:

Context

There is an unmet need to discover new treatments for Alzheimer's disease. This study determined the anti-acetylcholinesterase (AChE) activity, DPPH free radical scavenging and antioxidant properties of Carpolobia lutea G. Don (Polygalaceae).

Objective

The objective of this study is to quantify C. lutea anti-AChE, DPPH free radical scavenging, and antioxidant activities and cell cytotoxicity.

Materials and methods

Plant stem, leaves and roots were subjected to sequential solvent extractions, and screened for anti-AChE activity across a concentration range of 0.02-200??g/mL. Plant DPPH radical scavenging activity, reducing power, and total phenolic and flavonoid contents were determined, and cytotoxicity evaluated using human hepatocytes.

Results

Carpolobia lutea exhibited concentration-dependent anti-AChE activity. The most potent inhibitory activity for the stem was the crude ethanol extract and hexane stem fraction oil (IC₅₀?=?140??g/mL); for the leaves, the chloroform leaf fraction (IC₅₀?=?60??g/mL); and for roots, the methanol, ethyl acetate and aqueous root fractions (IC₅₀?=?0.3-3??g/mL). Dose-dependent free radical scavenging activity and reducing power were observed with increasing stem, leaf or root concentration. Total phenolic contents were the highest in the stem: ?632?mg gallic acid equivalents/g for a hexane stem fraction oil. Total flavonoid content was the highest in the leaves: ?297?mg quercetin equivalents/g for a chloroform leaf fraction. At 1??g/mL, only the crude ethanol extract oil was significantly cytotoxic to hepatocytes.

Discussion and conclusions

Carpolobia lutea possesses anti-AChE activity and beneficial antioxidant capacity indicative of its potential development as a treatment of Alzheimer's and other diseases characterized by a cholinergic deficit.

SUBMITTER: Nwidu LL

PROVIDER: S-EPMC6130458 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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Publications

Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea.

Nwidu Lucky Legbosi LL Elmorsy Ekramy E Thornton Jack J Wijamunige Buddhika B Wijesekara Anusha A Tarbox Rebecca R Warren Averil A Carter Wayne Grant WG

Pharmaceutical biology 20171201 1

<h4>Context</h4>There is an unmet need to discover new treatments for Alzheimer's disease. This study determined the anti-acetylcholinesterase (AChE) activity, DPPH free radical scavenging and antioxidant properties of Carpolobia lutea G. Don (Polygalaceae).<h4>Objective</h4>The objective of this study is to quantify C. lutea anti-AChE, DPPH free radical scavenging, and antioxidant activities and cell cytotoxicity.<h4>Materials and methods</h4>Plant stem, leaves and roots were subjected to seque ...[more]

PMID: 28629287

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Project description:Purpose:Acetylcholinesterase (AChE) inhibitors are used to treat Alzheimer's patients because they enhance cholinergic neurotransmission. It is urgent to find new and efficient inhibitors from natural sources, highly bioavailable with low or no toxicity. The plant kingdom is extremely rich in a variety of compounds that are potent AChE inhibitors: flavonoids and other phenolic compounds have been recognized as promising Alzheimer's treatment agents. In this study, in vitro acetylcholinesterase inhibition, antioxidant activities, and total flavonoid and phenolic contents of ethanol-water extracts from Quercus suber cork and corkback were evaluated. Methods:The acetylcholinesterase activity was determined by a colorimetric assay based on Ellman's methodology. The Folin-Ciocalteu colorimetric method was used for total phenolic content determination and the aluminium chloride method for the determination of total flavonoid content. Antioxidant activity assays were performed using the DPPH and FRAP assays. Results:The acetylcholinesterase inhibitory activity from Q. suber cork and corkback ethanol-water extracts was as follows: 62% inhibition with corkback extracts over 0.5?mg/mL and around 49% inhibition in cork extracts over 1.0?mg/mL extracts' concentration. Regarding the DPPH radical scavenging activity, the concentrations of cork and corkback ethanol-water extracts required for 50% DPPH inhibition (IC50) were 3.2??g/mL and 4.0-5.2??g/mL. Corkback extracts are less effective than Trolox standard (3.2??g/mL) but cork extracts showed the same free radical scavenging activity compared to Trolox. Cork and corkback extracts have antioxidant power of 750.9-775.4?mg TEAC/g extract and 1051.2-2052.4?mg TEAC/g extract, respectively, which are significantly higher than the ones obtained with Trolox: 19.6-21.0?mg TEAC/g extract (cork assays) and 57.4-66.3?mg TEAC/g extract (corkback assays). The amounts of total phenolic (TPC) and flavonoid (TFC) compounds were 8.7-32.3?mg GAE/g and 4.8-10.7?mg CE/g dry mass for cork and 5.4-5.7?mg GAE/g and 42.5?mg CE/g dry mass for corkback extracts, respectively, using catechin (CE) and GAE (gallic acid) as standards. Conclusion:These findings demonstrate the remarkable potential of these extracts as valuable source of antioxidants with interesting acetylcholinesterase inhibitory activity.

Dataset Information

Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea.

Context

Objective

Materials and methods

Results

Discussion and conclusions

Publications

Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea.

Similar Datasets

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