Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea.

ABSTRACT:

Context

There is an unmet need to discover new treatments for Alzheimer's disease. This study determined the anti-acetylcholinesterase (AChE) activity, DPPH free radical scavenging and antioxidant properties of Carpolobia lutea G. Don (Polygalaceae).

Objective

The objective of this study is to quantify C. lutea anti-AChE, DPPH free radical scavenging, and antioxidant activities and cell cytotoxicity.

Materials and methods

Plant stem, leaves and roots were subjected to sequential solvent extractions, and screened for anti-AChE activity across a concentration range of 0.02-200??g/mL. Plant DPPH radical scavenging activity, reducing power, and total phenolic and flavonoid contents were determined, and cytotoxicity evaluated using human hepatocytes.

Results

Carpolobia lutea exhibited concentration-dependent anti-AChE activity. The most potent inhibitory activity for the stem was the crude ethanol extract and hexane stem fraction oil (IC₅₀?=?140??g/mL); for the leaves, the chloroform leaf fraction (IC₅₀?=?60??g/mL); and for roots, the methanol, ethyl acetate and aqueous root fractions (IC₅₀?=?0.3-3??g/mL). Dose-dependent free radical scavenging activity and reducing power were observed with increasing stem, leaf or root concentration. Total phenolic contents were the highest in the stem: ?632?mg gallic acid equivalents/g for a hexane stem fraction oil. Total flavonoid content was the highest in the leaves: ?297?mg quercetin equivalents/g for a chloroform leaf fraction. At 1??g/mL, only the crude ethanol extract oil was significantly cytotoxic to hepatocytes.

Discussion and conclusions

Carpolobia lutea possesses anti-AChE activity and beneficial antioxidant capacity indicative of its potential development as a treatment of Alzheimer's and other diseases characterized by a cholinergic deficit.

SUBMITTER: Nwidu LL 

PROVIDER: S-EPMC6130458 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature