Quantification of Intrinsically Disordered Proteins: A Problem Not Fully Appreciated.

ABSTRACT: Protein quantification is essential in a great variety of biochemical assays, yet the inherent systematic errors associated with the concentration determination of intrinsically disordered proteins (IDPs) using classical methods are hardly appreciated. Routinely used assays for protein quantification, such as the Bradford assay or ultraviolet absorbance at 280 nm, usually seriously misestimate the concentrations of IDPs due to their distinct and variable amino acid composition. Therefore, dependable method(s) have to be worked out/adopted for this task. By comparison to elemental analysis as the gold standard, we show through the example of four globular proteins and nine IDPs that the ninhydrin assay and the commercial Qubit^TM Protein Assay provide reliable data on IDP quantity

SUBMITTER: Contreras-Martos S 

PROVIDER: S-EPMC6131523 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature