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Polyphenol profile by UHPLC-MS/MS, anti-glycation, antioxidant and cytotoxic activities of several samples of propolis from the northeastern semi-arid region of Brazil.
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ABSTRACT: Context
Propolis has promising biological activities. Propolis samples from the Northeast of Bahia, Brazil - sample A from Ribeira do Pombal and B, from Tucano - were investigated, with new information regarding their biological activities.Objective
This paper describes the chemical profile, antioxidant, anti-glycation and cytotoxic activities of these propolis samples.Material and methods
Ethanol extracts of these propolis samples (EEP) and their fractions were analyzed to determine total phenolic content (TPC); antioxidant capacity through DPPH•, FRAP and lipid peroxidation; anti-glycation activity, by an in vitro glucose (10?mg/mL) bovine serum albumine (1?mg/mL) assay, during 7?d; cytotoxic activity on cancer (SF295, HCT-116, OVCAR-8, MDA-MB435, MX-1, MCF7, HL60, JURKAT, MOLT-4, K562, PC3, DU145) and normal cell lines (V79) at 0.04-25??g/mL concentrations, for 72?h. The determination of primary phenols by ultra high-pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) and volatile organic compounds content by gas chromatography-mass spectrometry (GC-MS) were also performed.Results
The EEP polar fractions exhibited up to 90% protection against lipid peroxidation. The IC50 value for anti-glycation activity of EEP was between 16.5 and 19.2??g/mL, close to aminoguanidine (IC50?=?7.7??g/mL). The use of UHPLC-MS/MS and GC-MS allowed the identification of 12 bioactive phenols in the EEP and 24 volatile compounds, all already reported.Conclusions
The samples present good antioxidant/anti-glycation/cytotoxic activities and a plethora of biologically active compounds. These results suggest a potential role of propolis in targeting ageing and diseases associated with oxidative and carbonylic stress, aggregating value to them.
SUBMITTER: Xavier JA
PROVIDER: S-EPMC6131762 | biostudies-literature | 2017 Dec
REPOSITORIES: biostudies-literature
Publications
Polyphenol profile by UHPLC-MS/MS, anti-glycation, antioxidant and cytotoxic activities of several samples of propolis from the northeastern semi-arid region of Brazil.
Pharmaceutical biology 20171201 1
<h4>Context</h4>Propolis has promising biological activities. Propolis samples from the Northeast of Bahia, Brazil - sample A from Ribeira do Pombal and B, from Tucano - were investigated, with new information regarding their biological activities.<h4>Objective</h4>This paper describes the chemical profile, antioxidant, anti-glycation and cytotoxic activities of these propolis samples.<h4>Material and methods</h4>Ethanol extracts of these propolis samples (EEP) and their fractions were analyzed ...[more]
