Quantification of variation and the impact of biomass in targeted 16S rRNA gene sequencing studies.

ABSTRACT:

Background

Recent advances in sequencing technologies and bioinformatics tools have allowed for large-scale microbiome studies that are rapidly advancing medical research. However, small changes in technique or analysis can significantly alter the results and lead to conflicting findings. Quantifying the technical versus biological variation expected in targeted 16S rRNA gene sequencing studies and how this variation changes with input biomass is critical to guide meaningful interpretation of the current literature and plan future research.

Results

Data were compiled from 469 sequencing libraries across 19 separate targeted 16S rRNA gene sequencing runs over a 2.5-year time period. Following removal of contaminant sequences identified from negative controls, 244 samples reta

SUBMITTER: Bender JM 

PROVIDER: S-EPMC6131952 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature