Dataset Information

Quantification of desmosine and isodesmosine using MALDI-ion trap tandem mass spectrometry.

ABSTRACT: Desmosine (Des) and isodesmosine (Isodes), cross-linking amino acids in the biomolecule elastin, may be used as biomarkers for various pathological conditions associated with elastin degradation. The current study presents a novel approach to quantify Des and Isodes using matrix-assisted laser desorption ionization (MALDI)-tandem mass spectrometry (MS2) in a linear ion trap coupled to a vacuum MALDI source. MALDI-MS2 analyses of Des and Isodes are performed using stable-isotope-labeled desmosine d4 (labeled-Des) as an internal standard in different biological fluids, such as urine and serum. The method demonstrated linearity over two orders of magnitude with a detection limit of 0.02 ng/?L in both urine and serum without enrichment prior to mass spectrometry, and relative standard deviation of

SUBMITTER: Rathod P

PROVIDER: S-EPMC6133720 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Quantification of desmosine and isodesmosine using MALDI-ion trap tandem mass spectrometry.

Rathod Pratikkumar P Kaur Manjeet M Ho Hsin-Pin HP Louis Marissa E ME Dhital Basant B Durlik Philip P Boutis Gregory S GS Mark Kevin J KJ Lee Jong I JI Chang Emmanuel J EJ

Analytical and bioanalytical chemistry 20180731 26

Desmosine (Des) and isodesmosine (Isodes), cross-linking amino acids in the biomolecule elastin, may be used as biomarkers for various pathological conditions associated with elastin degradation. The current study presents a novel approach to quantify Des and Isodes using matrix-assisted laser desorption ionization (MALDI)-tandem mass spectrometry (MS<sup>2</sup>) in a linear ion trap coupled to a vacuum MALDI source. MALDI-MS<sup>2</sup> analyses of Des and Isodes are performed using stable-iso ...[more]

PMID: 30062515

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Project description:Nitromethane is a volatile organic compound categorized as a Group 2B carcinogen by the International Agency for Research on Cancer. It has been detected in mainstream cigarette smoke, but few reliable methods have been reported for accurate quantification. We developed, a sensitive, selective, fully validated method for the targeted determination of nitromethane in mainstream tobacco smoke in ten U.S. domestic brands and two quality control materials (3R4F and CM6). The vapor phase portion of machine-generated cigarette mainstream smoke, under modified ISO 3308:2000 regime (ISO) and modified intense regime (HCI), from single cigarettes was collected using airtight polyvinylfluoride sampling bags. The bags' contents were extracted using methanol containing an isotopically labeled internal standard followed by gas chromatography-tandem mass spectrometry. This approach is sufficiently sensitive to measure nitromethane levels in the nanogram range, with a method limit of detection of 72.3 ng/cig. Within-product variability estimated from the replicate analysis of 10 products ranged from 4.6%-16.3% (n = 6) over the two different smoking regimes, and method reproducibility estimated from two products used as quality control materials (3R4F and CM6) yielded intermediate precision values ranging from 16.6 to 20.8% (n = 20). Under HCI, nitromethane yields in machine-generated cigarette smoke from ten different domestic cigarette products ranged from 3.2 to 12 μg/cig; under ISO yields ranged from 1.6 to 4.9 μg/cig under standardized smoking machine conditions. Nitromethane yields are related to both the smoke regime (blocking of vent holes, puff duration and puff volume) and the heterogeneity of tobacco mixtures. This method provides a selective and fully validated technique to accurately quantify nitromethane in mainstream cigarette smoke, with minimal waste generation. It is an improvement over previous methods with regards to specificity, throughput, and simplicity of the sample collection process.

Dataset Information

Quantification of desmosine and isodesmosine using MALDI-ion trap tandem mass spectrometry.

Publications

Quantification of desmosine and isodesmosine using MALDI-ion trap tandem mass spectrometry.

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