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Quantification of desmosine and isodesmosine using MALDI-ion trap tandem mass spectrometry.


ABSTRACT: Desmosine (Des) and isodesmosine (Isodes), cross-linking amino acids in the biomolecule elastin, may be used as biomarkers for various pathological conditions associated with elastin degradation. The current study presents a novel approach to quantify Des and Isodes using matrix-assisted laser desorption ionization (MALDI)-tandem mass spectrometry (MS2) in a linear ion trap coupled to a vacuum MALDI source. MALDI-MS2 analyses of Des and Isodes are performed using stable-isotope-labeled desmosine d4 (labeled-Des) as an internal standard in different biological fluids, such as urine and serum. The method demonstrated linearity over two orders of magnitude with a detection limit of 0.02 ng/?L in both urine and serum without enrichment prior to mass spectrometry, and relative standard deviation of

SUBMITTER: Rathod P 

PROVIDER: S-EPMC6133720 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Quantification of desmosine and isodesmosine using MALDI-ion trap tandem mass spectrometry.

Rathod Pratikkumar P   Kaur Manjeet M   Ho Hsin-Pin HP   Louis Marissa E ME   Dhital Basant B   Durlik Philip P   Boutis Gregory S GS   Mark Kevin J KJ   Lee Jong I JI   Chang Emmanuel J EJ  

Analytical and bioanalytical chemistry 20180731 26


Desmosine (Des) and isodesmosine (Isodes), cross-linking amino acids in the biomolecule elastin, may be used as biomarkers for various pathological conditions associated with elastin degradation. The current study presents a novel approach to quantify Des and Isodes using matrix-assisted laser desorption ionization (MALDI)-tandem mass spectrometry (MS<sup>2</sup>) in a linear ion trap coupled to a vacuum MALDI source. MALDI-MS<sup>2</sup> analyses of Des and Isodes are performed using stable-iso  ...[more]

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