Microfluidic-enabled quantitative measurements of insulin release dynamics from single islets of Langerhans in response to 5-palmitic acid hydroxy stearic acid.

ABSTRACT: Proper release of insulin from pancreatic islets of Langerhans is essential for maintaining glucose homeostasis. For full efficacy, both the pattern and the amount of hormone release are critical. It is therefore important to understand how insulin levels are secreted from single islets in both a quantitative fashion and in a manner that resolves temporal dynamics. In this study, we describe a microfluidic analytical system that can both quantitatively monitor insulin secretion from single islets while simultaneously maintaining high temporal sampling to resolve dynamics of release. We have applied this system to determine the acute and chronic effects of a recently-identified lipid, 5-palmitic acid hydroxy stearic acid (5-PAHSA), which is a member of the fatty acid hydroxy fatty acid clas

SUBMITTER: Bandak B 

PROVIDER: S-EPMC6133761 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature