Unknown

Dataset Information

0

Role of the MAPK/cJun NH2-terminal kinase signaling pathway in starvation-induced autophagy.

ABSTRACT: Autophagy is required for cellular homeostasis and can determine cell viability in response to stress. It is established that MTOR is a master regulator of starvation-induced macroautophagy/autophagy, but recent studies have also implicated an essential role for the MAPK8/cJun NH2-terminal kinase 1 signal transduction pathway. We found that MAPK8/JNK1 and MAPK9/JNK2 were not required for autophagy caused by starvation or MTOR inhibition in murine fibroblasts and epithelial cells. These data demonstrate that MAPK8/9 has no required role in starvation-induced autophagy. We conclude that the role of MAPK8/9 in autophagy may be context-dependent and more complex than previously considered.

Abbreviations

AKT: thymoma viral proto-oncogene;ALB: albumin; ATG4: autophagy related 4; BCL2: B cell leukemia/lymphoma 2; BECN1: beclin 1, autophagy related; BNIP3: BCL2/adenovirus E1B interacting protein 3; CQ: chloroquine diphosphate; DMEM: Dulbecco's modified Eagle's medium; EDTA: ethylenediaminetetraacetic acid; EBSS: Earle's balanced salt solution; FBS: fetal bovine serum; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GFP: green fluorescent protein; HRAS: Harvey rat sarcoma virus oncogene; IgG: Immunoglobulin G; MAPK3/ERK1: mitogen-activated protein kinase 3; MAPK8/JNK1: mitogen-activated protein kinase 8; MAPK9/JNK2: mitogen-activated protein kinase 9; MAPK10/JNK3: mitogen-activated protein kinase 10; MAP1LC3B/LC3B: microtubule-associated protein 1 light chain 3 beta; MEFs: mouse embryonic fibroblasts; MTOR: mechanistic target of rapamycin kinase; RPS6KB1/p70: ribosomal protein S6 kinase, polypeptide 1; PPARA: peroxisome proliferator activated receptor alpha; SEM: standard error of the mean; SQSTM1/p62: sequestosome 1; TORC1: target of rapamycin complex 1; TORC2: target of rapamycin complex 2; TRP53: transforming related protein 53; TUBA: tubulin alpha; UV: ultraviolet; WT: wild-type.

SUBMITTER: Barutcu SA 

PROVIDER: S-EPMC6135577 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Role of the MAPK/cJun NH<sub>2</sub>-terminal kinase signaling pathway in starvation-induced autophagy.

Barutcu Seda Avcioglu SA   Girnius Nomeda N   Vernia Santiago S   Davis Roger J RJ  

Autophagy 20180817 9

Autophagy is required for cellular homeostasis and can determine cell viability in response to stress. It is established that MTOR is a master regulator of starvation-induced macroautophagy/autophagy, but recent studies have also implicated an essential role for the MAPK8/cJun NH<sub>2</sub>-terminal kinase 1 signal transduction pathway. We found that MAPK8/JNK1 and MAPK9/JNK2 were not required for autophagy caused by starvation or MTOR inhibition in murine fibroblasts and epithelial cells. Thes  ...[more]

Similar Datasets

Unknown The cJUN NH2-terminal kinase (JNK) signaling pathway promotes genome stability and prevents tumor initiation.
| S-EPMC5984035 | biostudies-literature
Unknown The cJUN NH2-terminal kinase (JNK) pathway contributes to mouse mammary gland remodeling during involution.
| S-EPMC6143629 | biostudies-literature
Unknown Pharmacological c-Jun NH<sub>2</sub>-Terminal Kinase (JNK) Pathway Inhibition Reduces Severity of Spinal Muscular Atrophy Disease in Mice.
| S-EPMC6131195 | biostudies-literature
Unknown Inactivation of Hippo and cJun-N-terminal Kinase (JNK) signaling mitigate FUS mediated neurodegeneration in vivo.
| S-EPMC9277911 | biostudies-literature
Unknown Activation of the c-Jun NH<sub>2</sub>-terminal kinase pathway by coronavirus infectious bronchitis virus promotes apoptosis independently of c-Jun.
| S-EPMC5870581 | biostudies-literature
Unknown Activation of p42 mitogen-activated protein kinase (MAPK), but not c-Jun NH(2)-terminal kinase, induces phosphorylation and stabilization of MAPK phosphatase XCL100 in Xenopus oocytes.
| S-EPMC65641 | biostudies-literature
Transcriptomics Targets of c-Jun NH(2)-terminal kinase 2-mediated tumor growth regulation
2003-07-16 | GSE508 | GEO
Unknown IL-6 Inhibits Starvation-induced Autophagy via the STAT3/Bcl-2 Signaling Pathway.
| S-EPMC4637890 | biostudies-literature
Unknown cJun N-terminal kinase (JNK) phosphorylation of serine 36 is critical for p66Shc activation.
| S-EPMC4751440 | biostudies-literature
Unknown The Drosophila Ral GTPase regulates developmental cell shape changes through the Jun NH(2)-terminal kinase pathway.
| S-EPMC3206575 | biostudies-literature