Label-free 3D-CLEM Using Endogenous Tissue Landmarks.
Ontology highlight
ABSTRACT:
SUBMITTER: Luckner M
PROVIDER: S-EPMC6137285 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Publications
Label-free 3D-CLEM Using Endogenous Tissue Landmarks.
Luckner Manja M Burgold Steffen S Filser Severin S Scheungrab Maximilian M Niyaz Yilmaz Y Hummel Eric E Wanner Gerhard G Herms Jochen J
iScience 20180720
Emerging 3D correlative light and electron microscopy approaches enable studying neuronal structure-function relations at unprecedented depth and precision. However, established protocols for the correlation of light and electron micrographs rely on the introduction of artificial fiducial markers, such as polymer beads or near-infrared brandings, which might obscure or even damage the structure under investigation. Here, we report a general applicable "flat embedding" preparation, enabling high- ...[more]
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