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VIG-1 is required for maintenance of genome stability in Caenorhabditis elegans.

ABSTRACT: To explore the function of VIG-1 in Caenorhabditis elegans, we analyzed the phenotypes of two vig-1 deletion mutants: vig-1(tm3383) and vig-1(ok2536). Both vig-1 mutants exhibited phenotypes associated with genome instability, such as a high incidence of males (Him) and increased embryonic lethality. These phenotypes became more evident in succeeding generations, implying that the germline of vig-1 accumulates DNA damage over generations. To examine whether vig-1 causes a defect in the DNA damage response, we treated worms with UV or camptothecin, a specific topoisomerase I inhibitor. We observed that the embryonic survival of the vig-1 mutants was reduced compared with that of the wild-type worms. Our results thus suggest that VIG-1 is required for maintaining genome stability in response to endogenous and exogenous genotoxic stresses.

SUBMITTER: Vasamsetti BMK

PROVIDER: S-EPMC6138327 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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VIG-1 is required for maintenance of genome stability in Caenorhabditis elegans.

Vasamsetti Bala Murali Krishna BMK Park Yang-Seo YS Cho Nam Jeong NJ

Animal cells and systems 20180524 3

To explore the function of VIG-1 in Caenorhabditis elegans, we analyzed the phenotypes of two vig-1 deletion mutants: vig-1(tm3383) and vig-1(ok2536). Both vig-1 mutants exhibited phenotypes associated with genome instability, such as a high incidence of males (Him) and increased embryonic lethality. These phenotypes became more evident in succeeding generations, implying that the germline of vig-1 accumulates DNA damage over generations. T ...[more]

PMID: 30460098

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