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Small RNA and degradome sequencing used to elucidate the basis of tolerance to salinity and alkalinity in wheat.

ABSTRACT: BACKGROUND:Soil salinity and/or alkalinity impose a major constraint over crop yield and quality. An understanding of the molecular basis of the plant response to these stresses could inform the breeding of more tolerant varieties. The bread wheat cultivar SR3 exhibits an enhanced level of salinity tolerance, while SR4 is distinguished by its superior tolerance of alkalinity. RESULTS:The small RNA and degradome sequencing was used to explore the miRNAs and corresponding targets associated with the superior stress tolerance of the SR lines. An examination of the small RNA content of these two closely related lines revealed the presence of 98 known and 219 novel miRNA sequences. Degradome libraries were constructed in order to identify the targets of the miRNAs, leading to the identification of 58 genes targeted by 26 of the known miRNAs and 549 targeted by 65 of the novel ones. The function of two of the stress-responsive miRNAs was explored using virus-induced gene silencing. CONCLUSIONS:This analysis indicated that regulation mediated by both auxin and epigenetic modification can be important in determining both salinity and alkalinity tolerance, while jasmonate signaling and carbohydrate metabolism are important for salinity tolerance, as is proton transport for alkalinity tolerance.

SUBMITTER: Han H

PROVIDER: S-EPMC6139162 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Small RNA and degradome sequencing used to elucidate the basis of tolerance to salinity and alkalinity in wheat.

Han Huanan H Wang Qi Q Wei Lin L Liang Yu Y Dai Jiulan J Xia Guangmin G Liu Shuwei S

BMC plant biology 20180915 1

<h4>Background</h4>Soil salinity and/or alkalinity impose a major constraint over crop yield and quality. An understanding of the molecular basis of the plant response to these stresses could inform the breeding of more tolerant varieties. The bread wheat cultivar SR3 exhibits an enhanced level of salinity tolerance, while SR4 is distinguished by its superior tolerance of alkalinity.<h4>Results</h4>The small RNA and degradome sequencing was used to explore the miRNAs and corresponding targets as ...[more]

PMID: 30219055

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Project description:BackgroundSalinity-alkalinity stress is one of the major abiotic stresses affecting plant growth and development. γ-Aminobutyrate (GABA) is a non-protein amino acid that functions in stress tolerance. However, the interactions between cellular redox signaling and chlorophyll (Chl) metabolism involved in GABA-induced salinity-alkalinity stress tolerance in plants remains largely unknown. Here, we investigated the role of GABA in perceiving and regulating chlorophyll biosynthesis and oxidative stress induced by salinity-alkalinity stress in muskmelon leaves. We also evaluated the effects of hydrogen peroxide (H2O2), glutathione (GSH), and ascorbate (AsA) on GABA-induced salinity-alkalinity stress tolerance.ResultsSalinity-alkalinity stress increased malondialdehyde (MDA) content, relative electrical conductivity (REC), and the activities of superoxide dismutase (SOD), ascorbate peroxidase (APX) and dehydroascorbate reductase (DHAR). Salinity-alkalinity stress decreased shoot dry and fresh weight and leaf area, reduced glutathione and ascorbate (GSH and AsA) contents, activities of glutathione reductase (GR) and monodehydroascorbate reductase (MDAR). By contrast, pretreatment with GABA, H2O2, GSH, or AsA significantly inhibited these salinity-alkalinity stress-induced effects. The ability of GABA to relieve salinity-alkalinity stress was significantly reduced when the production of endogenous H2O2 was inhibited, but was not affected by inhibiting endogenous AsA and GSH production. Exogenous GABA induced respiratory burst oxidase homologue D (RBOHD) genes expression and H2O2 accumulation under normal conditions but reduced the H2O2 content under salinity-alkalinity stress. Salinity-alkalinity stress increased the accumulation of the chlorophyll synthesis precursors glutamate (Glu), δ-aminolevulinic acid (ALA), porphobilinogen (PBG), uroporphyrinogen III (URO III), Mg-protoporphyrin IX (Mg-proto IX), protoporphyrin IX (Proto IX), protochlorophyll (Pchl), thereby increasing the Chl content. Under salinity-alkalinity stress, exogenous GABA increased ALA content, but reduced the contents of Glu, PBG, URO III, Mg-proto IX, Proto IX, Pchl, and Chl. However, salinity-alkalinity stress or GABA treated plant genes expression involved in Chl synthesis had no consistent trends with Chl precursor contents.ConclusionsExogenous GABA elevated H2O2 may act as a signal molecule, while AsA and GSH function as antioxidants, in GABA-induced salinity-alkalinity tolerance. These factors maintain membrane integrity which was essential for the ordered chlorophyll biosynthesis. Pretreatment with exogenous GABA mitigated salinity-alkalinity stress caused excessive accumulation of Chl and its precursors, to avoid photooxidation injury.

Dataset Information

Small RNA and degradome sequencing used to elucidate the basis of tolerance to salinity and alkalinity in wheat.

Publications

Small RNA and degradome sequencing used to elucidate the basis of tolerance to salinity and alkalinity in wheat.

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