Ontology highlight
ABSTRACT:
SUBMITTER: Yamamoto K
PROVIDER: S-EPMC6140639 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Journal of pesticide science 20161101 4
cDNA encoding an unclassified glutathione <i>S</i>-transferase (GST) of the diamondback moth, <i>Plutella xylostella</i>, was cloned by reverse transcriptase-polymerase chain reaction. The resulting clone was sequenced and the amino acid sequence deduced, revealing 67%-73% identities with unclassified GSTs from other organisms. A recombinant protein was functionally overexpressed in <i>Escherichia coli</i> cells in a soluble form and purified to homogeneity. The enzyme was capable to catalyze th ...[more]