Ontology highlight
ABSTRACT:
SUBMITTER: Sanchez-Hernandez S
PROVIDER: S-EPMC6141704 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Sánchez-Hernández Sabina S Gutierrez-Guerrero Alejandra A Martín-Guerra Rocío R Cortijo-Gutierrez Marina M Tristán-Manzano María M Rodriguez-Perales Sandra S Sanchez Laura L Garcia-Perez Jose Luis JL Chato-Astrain Jesus J Fernandez-Valades Ricardo R Carrillo-Galvez Ana Belén AB Anderson Per P Montes Rosa R Real Pedro J PJ Martin Francisco F Benabdellah Karim K
Molecular therapy. Nucleic acids 20180818
Integration-defective lentiviral vectors (IDLVs) have become an important alternative tool for gene therapy applications and basic research. Unfortunately, IDLVs show lower transgene expression as compared to their integrating counterparts. In this study, we aimed to improve the expression levels of IDLVs by inserting the IS2 element, which harbors SARs and HS4 sequences, into their LTRs (SE-IS2-IDLVs). Contrary to our expectations, the presence of the IS2 element did not abrogate epigenetic sil ...[more]