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Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense.


ABSTRACT: Cysteine-rich receptor-like kinases (CRKs) are a large subfamily of plant receptor-like kinases that play a critical role in disease resistance in plants. However, knowledge about the CRK gene family in cotton and its function against Verticillium wilt (VW), a destructive disease caused by Verticillium dahliae that significantly reduces cotton yields is lacking. In this study, we identified a total of 30 typical CRKs in a Gossypium barbadense genome (GbCRKs). Eleven of these (>30%) are located on the A06 and D06 chromosomes, and 18 consisted of 9 paralogous pairs encoded in the A and D subgenomes. Phylogenetic analysis showed that the GbCRKs could be classified into four broad groups, the expansion of which has probably been driven by tandem duplication. Gene expression profiling of the GbCRKs in resistant and susceptible cotton cultivars revealed that a phylogenetic cluster of nine of the GbCRK genes were up-regulated in response to V. dahliae infection. Virus-induced gene silencing of each of these nine GbCRKs independently revealed that the silencing of GbCRK18 was sufficient to compromise VW resistance in G. barbadense. GbCRK18 expression could be induced by V. dahliae infection or jasmonic acid, and displayed plasma membrane localization. Therefore, our expression analyses indicated that the CRK gene family is differentially regulated in response to Verticillium infection, while gene silencing experiments revealed that GbCRK18 in particular confers VW resistance in G. barbadense.

SUBMITTER: Li TG 

PROVIDER: S-EPMC6141769 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals <i>GbCRK18</i> Confers Verticillium Wilt Resistance in <i>Gossypium barbadense</i>.

Li Ting-Gang TG   Zhang Dan-Dan DD   Zhou Lei L   Kong Zhi-Qiang ZQ   Hussaini Adamu S AS   Wang Dan D   Li Jun-Jiao JJ   Short Dylan P G DPG   Dhar Nikhilesh N   Klosterman Steven J SJ   Wang Bao-Li BL   Yin Chun-Mei CM   Subbarao Krishna V KV   Chen Jie-Yin JY   Dai Xiao-Feng XF  

Frontiers in plant science 20180911


Cysteine-rich receptor-like kinases (<i>CRKs</i>) are a large subfamily of plant receptor-like kinases that play a critical role in disease resistance in plants. However, knowledge about the <i>CRK</i> gene family in cotton and its function against Verticillium wilt (VW), a destructive disease caused by <i>Verticillium dahliae</i> that significantly reduces cotton yields is lacking. In this study, we identified a total of 30 typical <i>CRKs</i> in a <i>Gossypium barbadense</i> genome (<i>GbCRKs<  ...[more]

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