Ontology highlight
ABSTRACT:
SUBMITTER: Kleinpenning F
PROVIDER: S-EPMC6143282 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
ACS chemical biology 20180614 8
Mapping proteins at a specific subcellular location is essential to gaining detailed insight on local protein dynamics. We have developed an enzymatic strategy to label proteins on a subcellular level using arylamine N-acetyltransferase (NAT). The NAT enzyme activates an arylhydroxamic acid functionality into a nitrenium ion that reacts fast, covalently, and under neutral conditions with nucleophilic residues of neighboring proteins. The electron density on the aromatic ring proved important for ...[more]