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Quantitation of Intact Proteins in Human Plasma Using Top-Down Parallel Reaction Monitoring-MS.

ABSTRACT: Direct quantitation of proteins in complex biological matrices by mass spectrometry remains a challenge. Here, we describe a novel top-down parallel reaction monitoring-mass spectrometry (PRM-MS) assay, enabled by microflow LC-nanospray MS using a silicon microfluidic LC-MS chip. We demonstrated direct analysis of intact proteins such as somatropin in human plasma, achieving sensitivity (0.1-1.0 fmole) and speed (1-5 min) on par with enzyme-linked immunosorbent assay (ELISA).

SUBMITTER: Chen Y 

PROVIDER: S-EPMC6143396 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Quantitation of Intact Proteins in Human Plasma Using Top-Down Parallel Reaction Monitoring-MS.

Chen Yuchao Y   Mao Pan P   Wang Daojing D  

Analytical chemistry 20180907 18

Direct quantitation of proteins in complex biological matrices by mass spectrometry remains a challenge. Here, we describe a novel top-down parallel reaction monitoring-mass spectrometry (PRM-MS) assay, enabled by microflow LC-nanospray MS using a silicon microfluidic LC-MS chip. We demonstrated direct analysis of intact proteins such as somatropin in human plasma, achieving sensitivity (0.1-1.0 fmole) and speed (1-5 min) on par with enzyme-linked immunosorbent assay (ELISA). ...[more]

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