Quantitation of Intact Proteins in Human Plasma Using Top-Down Parallel Reaction Monitoring-MS.
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ABSTRACT:
SUBMITTER: Chen Y
PROVIDER: S-EPMC6143396 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Publications
Quantitation of Intact Proteins in Human Plasma Using Top-Down Parallel Reaction Monitoring-MS.
Chen Yuchao Y Mao Pan P Wang Daojing D
Analytical chemistry 20180907 18
Direct quantitation of proteins in complex biological matrices by mass spectrometry remains a challenge. Here, we describe a novel top-down parallel reaction monitoring-mass spectrometry (PRM-MS) assay, enabled by microflow LC-nanospray MS using a silicon microfluidic LC-MS chip. We demonstrated direct analysis of intact proteins such as somatropin in human plasma, achieving sensitivity (0.1-1.0 fmole) and speed (1-5 min) on par with enzyme-linked immunosorbent assay (ELISA). ...[more]
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