Ontology highlight
ABSTRACT:
SUBMITTER: Braselmann E
PROVIDER: S-EPMC6143402 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
Nature chemical biology 20180730 10
RNAs directly regulate a vast array of cellular processes, emphasizing the need for robust approaches to fluorescently label and track RNAs in living cells. Here, we develop an RNA imaging platform using the cobalamin riboswitch as an RNA tag and a series of probes containing cobalamin as a fluorescence quencher. This highly modular 'Riboglow' platform leverages different colored fluorescent dyes, linkers and riboswitch RNA tags to elicit fluorescence turn-on upon binding RNA. We demonstrate the ...[more]