Real-time tracking of single-molecule collagenase on native collagen and partially structured collagen-mimic substrates.
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ABSTRACT:
SUBMITTER: Froberg J
PROVIDER: S-EPMC6145137 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Publications
Real-time tracking of single-molecule collagenase on native collagen and partially structured collagen-mimic substrates.
Froberg James J Choi Woo-Sik WS Sedigh Abbas A Anajafi Tayebeh T Farmakes Jasmin J Yang Zhongyu Z Mallik Sanku S Srivastava D K DK Choi Yongki Y
Chemical communications (Cambridge, England) 20180901 73
The dynamic interactions of an individual matrix metalloproteinase-1 were imaged and monitored in the presence of either triple-helical or non-triple-helical, partially structured collagen-mimic substrates. The enzyme exhibited ten-fold increased catalytic turnover rates with the structurally modified substrate by skipping the triple-helix unwinding step during the catalytic pathway. ...[more]
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