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Generation of Drosophila sisRNAs by Independent Transcription from Cognate Introns.

ABSTRACT: Although stable intronic sequence RNAs (sisRNAs) are conserved in plants and animals, their functional significance is still unclear. We identify a pool of polyadenylated maternally deposited sisRNAs in Drosophila melanogaster. These sisRNAs can be generated by independent transcription from the cognate introns. The ovary-specific poly(A) polymerase Wispy mediates the polyadenylation of maternal sisRNAs and confers their stability as maternal transcripts. A developmentally regulated sisRNA sisR-3 represses the expression of a long noncoding RNA CR44148 and is required during development. Our results expand the pool of sisRNAs and suggest that sisRNAs perform regulatory functions during development in Drosophila.

SUBMITTER: Jia Ng SS

PROVIDER: S-EPMC6146417 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Generation of Drosophila sisRNAs by Independent Transcription from Cognate Introns.

Jia Ng Sharon Si SS Zheng Ruther Teo RT Osman Ismail I Pek Jun Wei JW

iScience 20180518

Although stable intronic sequence RNAs (sisRNAs) are conserved in plants and animals, their functional significance is still unclear. We identify a pool of polyadenylated maternally deposited sisRNAs in Drosophila melanogaster. These sisRNAs can be generated by independent transcription from the cognate introns. The ovary-specific poly(A) polymerase Wispy mediates the polyadenylation of maternal sisRNAs and confers their stability as maternal transcripts. A developmentally regulated sisRNA sisR- ...[more]

PMID: 30240754

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