Dataset Information

Promoted Angiogenesis and Osteogenesis by Dexamethasone-loaded Calcium Phosphate Nanoparticles/Collagen Composite Scaffolds with Microgroove Networks.

ABSTRACT: Reconstruction of large bone defects remains a clinical challenge because current approaches involving surgery and bone grafting often do not yield satisfactory outcomes. For artificial bone substitutes, angiogenesis plays a pivotal role to achieve the final success of newly regenerated bone. In this study, dexamethasone-loaded biphasic calcium phosphate nanoparticles/collagen composite scaffolds with several types of concave microgrooves were prepared for simultaneous promotion of angiogenesis and osteogenesis. Microgrooves in the scaffolds were supposed to guide the assembly of human umbilical vascular endothelial cells (HUVECs) into well aligned tubular structures, thus promoting rapid angiogenesis. The scaffolds were used for co-culture of HUVECs and human bone marrow-derived mesenchymal stem cells. Subcutaneous implantation in mice showed that more blood vessels and newly formed bone were observed in the microgrooved composite scaffolds than in the control scaffold. Scaffold bearing parallel microgrooves with a concave width of 290?µm and a convex ridge width of 352?µm showed the highest promotion effect on angiogenesis and osteogenesis among the parallelly microgrooved composite scaffolds. The scaffolds bearing a grid network had further superior promotion effect to the scaffolds bearing parallel microgrooves. The results indicated that microgrooves in the composite scaffolds facilitated angiogenesis and stimulated new bone formation. The microgrooved composite scaffolds should be useful for repairing of large bone defects.

SUBMITTER: Chen Y

PROVIDER: S-EPMC6147787 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Similar Datasets

Project description:Background/Objective: Tissue engineering involves scaffolds, cells and growth factors, among which growth factors have limited applications due to potential safety risks and high costs. Therefore, an alternative approach to exogenously induce osteogenesis is desirable. Considering that osteogenesis and angiogenesis are coupled, a system of human umbilical vein endothelial cells (HUVECs) and human bone mesenchymal stem cells (hBMSCs) coculture is more biologically adapted to the microenvironment in vivo and can mediate osteogenesis and angiogenesis via paracrine signalling. Hence, in this study, a HUVECs/hBMSCs coculture system with appropriate cell and medium proportions was established. The substrate for the coculture system was a 3D-printed composite bioceramic scaffold (β-TCP/CaSiO3) based on a previous study. The aim of this study was to explore the potential of this system for bone tissue engineering.MethodsBioactive ceramic scaffolds for tissue engineering were fabricated via a 3D Bioplotter™ system. The coculture system for in vitro and in vivo studies consisted of direct contact between HUVECs and hBMSCs cultured on the 3D-printed scaffolds.ResultsThe proportions of HUVECs/hBMSCs and medium components were determined by cell viability, and the coculture system showed negligible cytotoxicity. CD31 secreted by HUVECs formed strings, and cells tended to aggregate in island chain-like arrays. Real-time cell tracking showed that HUVECs were recruited by hBMSCs, and the integrin expression by HUVECs was upregulated. Ultimately, osteogenic and angiogenic marker gene expression and protein secretion were upregulated. Moreover, the obtained bone tissue engineering scaffolds could induce early osteogenic protein secretion and capillary tube formation in nude rats.ConclusionThese bone tissue engineering scaffolds without exogenous growth factors exhibited the ability to promote osteogenesis/angiogenesis.Translational potential of this articleThe fabricated 3D-printed bioactive ceramic scaffolds could provide mechanical, biodegradable and bioadaptive support for personalized bone regeneration. In addition, the bone tissue engineering scaffolds exhibited the ability to promote osteogenesis/angiogenesis without the addition of exogenous growth factors, thus mitigating safety risks. Although application of the HUVECs/hBMSCs coculture system might be a time-consuming process, further development of cord blood storage could be beneficial for multicell coculture.

Dataset Information

Promoted Angiogenesis and Osteogenesis by Dexamethasone-loaded Calcium Phosphate Nanoparticles/Collagen Composite Scaffolds with Microgroove Networks.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets