Dataset Information

Structural and Functional Properties Changes of ?-Conglycinin Exposed to Hydroxyl Radical-Generating Systems.

ABSTRACT: The objective of the present study was to examine the structural and functional changes of ?-conglycinin exposed to oxidizing radicals produced by FeCl?/H?O?/ascorbic acid hydroxyl radical-generating system (HRGS) for 3 h at room temperature. Increasing H?O? concentrations resulted in a loss of histidine residues, lysine residues, and available lysine, which was accompanied by the formation of protein carbonyls and disulphide bonds (p < 0.05). Changes in secondary structure, surface hydrophobicity, and intrinsic fluorescence indicated that hydroxyl radicals had induced protein unfolding and conformational alterations. Results from SDS-PAGE implied that a small amount of protein cross-linkages produced by oxidative incubation. The emulsifying properties of ?-conglycinin were gradually improved with the increasing extent of oxidation. The structural changes above contributed to the reduction of potential allergenicity of ?-conglycinin, as verified by specific ELISA analysis. These results suggest that moderate oxidation could partially improve the protein functional properties and reduced the potential allergy of protein, providing guidance for effective use of moderately oxidized soy protein in the industry.

SUBMITTER: Xu J

PROVIDER: S-EPMC6150227 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature

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Structural and Functional Properties Changes of β-Conglycinin Exposed to Hydroxyl Radical-Generating Systems.

Xu Jing J Chen Zijing Z Han Dong D Li Yangyang Y Sun Xiaotong X Wang Zhongjiang Z Jin Hua H

Molecules (Basel, Switzerland) 20171103 11

The objective of the present study was to examine the structural and functional changes of β-conglycinin exposed to oxidizing radicals produced by FeCl₃/H₂O₂/ascorbic acid hydroxyl radical-generating system (HRGS) for 3 h at room temperature. Increasing H₂O₂ concentrations resulted in a loss of histidine residues, lysine residues, and available lysine, which was accompanied by the formation of protein carbonyls and disulphide bonds (<i>p</i> < 0.05). Changes in secondary structure, surface hydro ...[more]

PMID: 29099771

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Project description:Advanced oxidation treatment processes used in various applications to treat contaminated soil, water, and groundwater involve powerful radical intermediates, including hydroxyl radicals (•OH). Inefficiency in •OH-driven treatment systems involves scavenging reactions where •OH react with non-target species in the aqueous and solid phases. Here, •OH were generated in iron (Fe)- and UV-activated hydrogen peroxide (Fe-AHP, UV-AHP) systems where the loss of rhodamine B served as a quantitative metric for •OH activity. Kinetic analysis methods were developed to estimate the specific •OH surface scavenging rate constant (k≡S). In the Fe-AHP system, k≡S for silica (2.85 × 106 1/m2 × s) and alumina (3.92 × 106 1/m2 × s) were similar. In the UV-AHP system, estimates of k≡S for silica (4.50 × 106 1/m2 × s) and alumina (7.45 × 106 1/m2 × s) were higher. k≡S for montmorillonite (MMT) in the UV-AHP system was ≤4.22 × 105 1/m2 × s. Overall, k≡S,silica ∼ k≡S, alumina > k≡S,MMT indicating k≡S is mineral specific. Radical scavenging was dominated by surface scavenging at 10-50 g/L silica, alumina, or MMT, in both Fe-AHP and UV-AHP systems. The experimentally-derived surface •OH scavenging rate constants were extended to in-situ chemical oxidation (ISCO) treatment conditions to contrast •OH reaction rates with contaminant and aqueous phase reactants found in aquifer systems. •OH reaction was dominated by solid surfaces comprised of silica, alumina, and montmorillonite minerals relative to •OH reaction with trichloroethylene, the target compound, and H2O2, a well-documented radical scavenger. These results indicate that solid mineral surfaces play a key role in limiting the degradation rate of contaminants found in soil and groundwater, and the overall treatment efficiency in ISCO systems. The aggressive •OH scavenging measured was partially attributed to the relative abundance of scavenging sites on mineral surfaces.

Dataset Information

Structural and Functional Properties Changes of ?-Conglycinin Exposed to Hydroxyl Radical-Generating Systems.

Publications

Structural and Functional Properties Changes of β-Conglycinin Exposed to Hydroxyl Radical-Generating Systems.

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