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Citrullination Inactivates Nicotinamide- N-methyltransferase.


ABSTRACT: Nicotinamide- N-methyltransferase (NNMT) catalyzes the irreversible methylation of nicotinamide (NAM) to form N-methyl nicotinamide using S-adenosyl methionine as a methyl donor. NNMT is implicated in several chronic disease conditions, including cancers, kidney disease, cardiovascular disease, and Parkinson's disease. Although phosphorylation of NNMT in gastric tumors is reported, the functional effects of this post-translational modification has not been investigated. We previously reported that citrullination of NNMT by Protein Arginine Deiminases abolished its methyltransferase activity. Herein, we investigate the mechanism of inactivation. Using tandem mass spectrometry, we identified three sites of citrullination in NNMT. With this information in hand, we used a combination of site-directed mutagenesis, kinetics, and circular dichoism experiments to demonstrate that citrullination of R132 leads to a structural perturbation that ultimately promotes NNMT inactivation.

SUBMITTER: Nemmara VV 

PROVIDER: S-EPMC6150842 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Citrullination Inactivates Nicotinamide- N-methyltransferase.

Nemmara Venkatesh V VV   Tilvawala Ronak R   Salinger Ari J AJ   Miller Lacey L   Nguyen Son Hong SH   Weerapana Eranthie E   Thompson Paul R PR  

ACS chemical biology 20180808 9


Nicotinamide- N-methyltransferase (NNMT) catalyzes the irreversible methylation of nicotinamide (NAM) to form N-methyl nicotinamide using S-adenosyl methionine as a methyl donor. NNMT is implicated in several chronic disease conditions, including cancers, kidney disease, cardiovascular disease, and Parkinson's disease. Although phosphorylation of NNMT in gastric tumors is reported, the functional effects of this post-translational modification has not been investigated. We previously reported th  ...[more]

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