Cell-wall dyes interfere with Cryptococcus neoformans melanin deposition.
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ABSTRACT:
SUBMITTER: Perez-Dulzaides R
PROVIDER: S-EPMC6152417 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
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Cell-wall dyes interfere with Cryptococcus neoformans melanin deposition.
Perez-Dulzaides Ricardo R Camacho Emma E Cordero Radames J B RJB Casadevall Arturo A
Microbiology (Reading, England) 20180625 8
Melanization is an intrinsic characteristic of many fungal species, but details of this process are poorly understood because melanins are notoriously difficult pigments to study. While studying the binding of cell-wall dyes, Eosin Y or Uvitex, to melanized and non-melanized Cryptococcus neoformans cells we noted that melanization leads to reduced fluorescence intensity, suggesting that melanin interfered with dye binding to the cell wall. The growth of C. neoformans in melanizing conditions wit ...[more]
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