Antimicrobial Susceptibility and Enterotoxin-Encoding Genes in Staphylococcus spp. Recovered from Kitchen Equipment from a University Hospital in Rio de Janeiro, Brazil.

ABSTRACT: This study was conducted to determine the occurrence of antimicrobial resistance and enterotoxin-encoding genes (EEGs) in Staphylococcus spp. recovered from equipment used to prepare hospital meals, in a university hospital in Rio de Janeiro, Brazil. Sixty samples were collected from semi-industrial equipment (one blender and one mixer) in the hospital's kitchen. Resistance genes and SCCmec types were detected by PCR. From the 40 isolates of Staphylococcus spp. identified, 8 were Staphylococcus aureus. Thirty-two (80%) Staphylococcus spp. isolates were resistant to at least one antimicrobial agent. Resistance genetic determinants were detected: erm gene (Staphylococcus epidermidis [n?=?2]; Staphylococcus hominis [n?=?1]), mecA gene (S. epidermidis [n?=?2]), and aa(6')-aph(2'') gene (Staphylococcus caprae [n?=?1], S. epidermidis [n?=?2], S. hominis [n?=?1], Staphylococcus pausteri [n?=?1], Staphylococcus simulans [n?=?1], and Staphylococcus warneri [n?=?1]). The presence of at least one EEG in 83% (n?=?33) of the isolates was identified. Two strains of S. epidermidis were methicillin-resistant S. epidermidis (MRSE) and harboring SCCmec type IV. Staphylococcus spp. contaminated some hospital kitchen's equipment, indicating that hygiene procedures should be improved. Results also indicate that meals can be a vehicle to disseminate multiresistant Staphylococcus spp., including MRSE, and Staphylococcus with EEGs.

SUBMITTER: Miyahira RF 

PROVIDER: S-EPMC6154758 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature