Ontology highlight
ABSTRACT:
SUBMITTER: Cai Y
PROVIDER: S-EPMC6156062 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Cai Yumei Y Yang Weiqun W Pan Mingmeng M Wang Chaoyang C Wu Wenyi W Zhu Shize S
Medicine 20180901 37
To study the effect of knocking down wingless-related MMTV integration site 2 (Wnt2) expression by RNAi on the growth and signaling pathways of ex vitro-cultured keloid fibroblasts (KFB).Human KFB were isolated from 10 keloid patient specimens. The KFB cells were then transfected with 4 pairs of small interfering RNA (siRNA) targeting human Wnt2, respectively. Reverse transcriptase-polymerase chain reaction and Western blot analysis were conducted to verify the knock down of Wnt2, and the expres ...[more]