Ontology highlight
ABSTRACT:
SUBMITTER: Gao S
PROVIDER: S-EPMC6156606 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Gao Shengxian S Feng Sumin S Ning Shaokai S Liu Jingyan J Zhao Huayu H Xu Yixi Y Shang Jinfeng J Li Kejiao K Li Qing Q Guo Rong R Xu Dongyi D
Nature communications 20180925 1
53BP1 with its downstream proteins, RIF1, PTIP and REV7, antagonizes BRCA1-dependent homologous recombination (HR) and promotes non-homologous end joining (NHEJ) in an unclear manner. Here we show that REV7 forms a complex with two proteins, FAM35A and C20ORF196. We demonstrate that FAM35A preferentially binds single-strand DNA (ssDNA) in vitro, and is recruited to DSBs as a complex with C20ORF196 and REV7 downstream of RIF1 in vivo. Epistasis analysis shows that both proteins act in the same pa ...[more]