Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes.
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ABSTRACT:
SUBMITTER: Jia Z
PROVIDER: S-EPMC6157888 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Publications
Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes.
Jia Zhiming Z Guo Hailin H Xie Hua H Bao Xingqi X Huang Yichen Y Yang Ganggang G Chen Fang F
PloS one 20180926 9
Cell sheet engineering has recently emerged as a promising strategy for scaffold-free tissue engineering. However, the primary method of harvesting cell sheets using temperature-responsive dishes has potential limitations. Here we report a novel cell sheet technology based on a coculture system in which SMCs are cocultured with EPCs on common polystyrene dishes. We found that an intact and highly viable cell sheet could be harvested using mechanical methods when SMCs and EPCs were cocultured on ...[more]
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