Unknown

Dataset Information

0

Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes.


ABSTRACT: Cell sheet engineering has recently emerged as a promising strategy for scaffold-free tissue engineering. However, the primary method of harvesting cell sheets using temperature-responsive dishes has potential limitations. Here we report a novel cell sheet technology based on a coculture system in which SMCs are cocultured with EPCs on common polystyrene dishes. We found that an intact and highly viable cell sheet could be harvested using mechanical methods when SMCs and EPCs were cocultured on common polystyrene dishes at a ratio of 6:1 for 5 to 6 days; the method is simple, cost-effective and highly repeatable. Moreover, the cocultured cell sheet contained capillary-like networks and could secrete a variety of angiogenic factors. Finally, in vivo studies proved that the cocultured cell sheets were more favorable for the fabrication of vascularized smooth muscle tissues compared to single SMC sheets. This study provides a promising avenue for smooth muscle tissue engineering.

SUBMITTER: Jia Z 

PROVIDER: S-EPMC6157888 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

altmetric image

Publications

Harvesting prevascularized smooth muscle cell sheets from common polystyrene culture dishes.

Jia Zhiming Z   Guo Hailin H   Xie Hua H   Bao Xingqi X   Huang Yichen Y   Yang Ganggang G   Chen Fang F  

PloS one 20180926 9


Cell sheet engineering has recently emerged as a promising strategy for scaffold-free tissue engineering. However, the primary method of harvesting cell sheets using temperature-responsive dishes has potential limitations. Here we report a novel cell sheet technology based on a coculture system in which SMCs are cocultured with EPCs on common polystyrene dishes. We found that an intact and highly viable cell sheet could be harvested using mechanical methods when SMCs and EPCs were cocultured on  ...[more]

Similar Datasets

| S-EPMC7482816 | biostudies-literature
| S-EPMC6456548 | biostudies-literature
| S-EPMC5568633 | biostudies-literature
| S-EPMC3249499 | biostudies-literature
| S-EPMC5020645 | biostudies-literature
| S-EPMC5984560 | biostudies-literature
| S-EPMC6972683 | biostudies-literature
| S-EPMC6986347 | biostudies-literature
| S-EPMC7719137 | biostudies-literature
| S-EPMC7289836 | biostudies-literature