Dataset Information

Cloning and Characterization of TpNRAMP3, a Metal Transporter From Polish Wheat (Triticum polonicum L.).

ABSTRACT: Essential transition metals and non-essential metals often co-exist in arable soils. In plants, some transition metal transporters, such as the natural resistance-associated macrophage proteins (NRAMPs), poorly selectively transport metals with similar chemical properties whether they are essential or non-essential. In this study, a member of the NRAMP transporter family, TpNRAMP3, was identified from dwarf Polish wheat (Triticum polonicum L.). TpNRAMP3 encodes a plasma membrane-localized protein and was highly expressed in leaf blades and roots at the jointing and booting stages, and in the first nodes at the grain filling stage. Expression of TpNRAMP3 increased sensitivity to Cd and Co, but not Zn, and increased the Cd and Co concentrations in yeast. TpNRAMP3 expression in Arabidopsis increased concentrations of Cd, Co, and Mn, but not Fe or Zn, in roots, shoots, and whole plant. However, TpNRAMP3 did not affect translocation of Cd, Co, or Mn from roots to shoots. These results suggest that TpNRAMP3 is a transporter for Cd, Co, and Mn accumulation, but not for Fe or Zn. However, Cd and Co are non-essential toxic metals; selective genetic manipulation of TpNRAMP3 will help breed low Cd- and Co-accumulating cultivars.

SUBMITTER: Peng F

PROVIDER: S-EPMC6158329 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Cloning and Characterization of TpNRAMP3, a Metal Transporter From Polish Wheat (Triticum polonicum L.).

Peng Fan F Wang Chao C Cheng Yiran Y Kang Houyang H Fan Xing X Sha Lina L Zhang Haiqin H Zeng Jian J Zhou Yonghong Y Wang Yi Y

Frontiers in plant science 20180920

Essential transition metals and non-essential metals often co-exist in arable soils. In plants, some transition metal transporters, such as the natural resistance-associated macrophage proteins (NRAMPs), poorly selectively transport metals with similar chemical properties whether they are essential or non-essential. In this study, a member of the NRAMP transporter family, TpNRAMP3, was identified from dwarf Polish wheat (Triticum polonicum L.). TpNRAMP3 encodes a plasma membrane-lo ...[more]

PMID: 30294336

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Project description:BACKGROUND: Argonaute proteins are key components of RNA interference (RNAi), playing important roles in RNA-directed gene silencing. Various classes of Argonaute genes have been identified from plants and might be involved in developmental regulation. However, little is known about these genes in wheat (Triticum aestivum). RESULTS: In this study, two full-length cDNAs of Argonaute were cloned from wheat, designated as TaAGO1b and TaAGO4. The cDNA of TaAGO1b is 3273 bp long and encodes 868 amino acids, with a predicted molecular weight of ~97.78 kDa and pI of 9.29. The 3157-bp TaAGO4 encodes 916 amino acids, with a molecular mass of 102.10 kDa and pI of 9.12. Genomics analysis showed that TaAGO1b and TaAGO4 contain 20 and 18 introns, respectively. Protein structural analysis demonstrated that typical PAZ and PIWI domains were found in both TaAGO1b and TaAGO4. From the highly conserved PIWI domains, we detected conserved Asp-Asp-His (DDH) motifs that function as a catalytic triad and have critical roles during the process of sequence-specific cleavage in the RNAi machinery. Structural modelling indicated that both TaAGOs can fold to a specific ?/? structure. Moreover, the three aligned DDH residues are spatially close to each other at the "slicer" site of the PIWI domain. Expression analysis indicated that both genes are ubiquitously expressed in vegetative and reproductive organs, including the root, stem, leaf, anther, ovule, and seed. However, they are differentially expressed in germinating endosperm tissues. We were interested to learn that the two TaAGOs are also differentially expressed in developing wheat plants and that their expression patterns are variously affected by vernalization treatment. Further investigation revealed that they can be induced by cold accumulation during vernalization. CONCLUSIONS: Two putative wheat Argonaute genes, TaAGO1b and TaAGO4, were cloned. Phylogenetic analysis, prediction of conserved domains and catalytic motifs, and modelling of their protein structures suggested that they encode functional Argonaute proteins. Temporal and spatial expression analyses indicated that these genes are potentially involved in developmental regulation of wheat plants.

Dataset Information

Cloning and Characterization of TpNRAMP3, a Metal Transporter From Polish Wheat (Triticum polonicum L.).

Publications

Cloning and Characterization of <i>TpNRAMP3</i>, a Metal Transporter From Polish Wheat (<i>Triticum polonicum</i> L.).

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