Dataset Information

Low-Frequency Blood Group Antigens in Switzerland.

ABSTRACT: Background:High-frequency blood group antigens (HFA) are present in >90% of the human population, according to some reports even in >99% of individuals. Therefore, patients lacking HFA may become challenging for transfusion support because compatible blood is hardly found, and if the patient carries alloantibodies, the cross-match will be positive with virtual every red cell unit tested. Methods:In this study, we applied high-throughput blood group SNP genotyping on >37,000 Swiss blood donors, intending to identify homozygous carriers of low-frequency blood group antigens (LFA). Results:326 such individuals were identified and made available to transfusion specialists for future support of patients in need of rare blood products. Conclusion:Thorough comparison of minor allele frequencies using population genetics revealed heterogeneity of allele distributions among Swiss blood donors which may be explained by the topographical and cultural peculiarities of Switzerland. Moreover, geographically localized donor subpopulations are described which contain above-average numbers of individuals carrying rare blood group genotypes.

SUBMITTER: Gassner C

PROVIDER: S-EPMC6158591 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Low-Frequency Blood Group Antigens in Switzerland.

Gassner Christoph C Degenhardt Frauke F Meyer Stefan S Vollmert Caren C Trost Nadine N Neuenschwander Kathrin K Merki Yvonne Y Portmann Claudia C Sigurdardottir Sonja S Zorbas Antigoni A Engström Charlotte C Gottschalk Jochen J Amar El Dusouqui Soraya S Waldvogel-Abramovski Sophie S Rigal Emmanuel E Tissot Jean-Daniel JD Tinguely Caroline C Mauvais Simon M SM Sarraj Amira A Bessero Daniel D Stalder Michele M Infanti Laura L Buser Andreas A Sigle Jörg J Weingand Tina T Castelli Damiano D Braisch Monica C MC Thierbach Jutta J Heer Sonja S Schulzki Thomas T Krawczak Michael M Franke Andre A Frey Beat M BM

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie 20180710 4

<h4>Background</h4>High-frequency blood group antigens (HFA) are present in >90% of the human population, according to some reports even in >99% of individuals. Therefore, patients lacking HFA may become challenging for transfusion support because compatible blood is hardly found, and if the patient carries alloantibodies, the cross-match will be positive with virtual every red cell unit tested.<h4>Methods</h4>In this study, we applied high-throughput blood group SNP genotyping on >37,000 Swiss ...[more]

PMID: 30283273

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Project description:UnlabelledHuman noroviruses are the dominant cause of outbreaks of gastroenteritis around the world. Human noroviruses interact with the polymorphic human histo-blood group antigens (HBGAs), and this interaction is thought to be important for infection. Indeed, synthetic HBGAs or HBGA-expressing enteric bacteria were shown to enhance norovirus infection in B cells. A number of studies have found a possible relationship between HBGA type and norovirus susceptibility. The genogroup II, genotype 4 (GII.4) noroviruses are the dominant cluster, evolve every other year, and are thought to modify their binding interactions with different HBGA types. Here we show high-resolution X-ray crystal structures of the capsid protruding (P) domains from epidemic GII.4 variants from 2004, 2006, and 2012, cocrystallized with a panel of HBGA types (H type 2, Lewis Y, Lewis B, Lewis A, Lewis X, A type, and B type). Many of the HBGA binding interactions were found to be complex, involving capsid loop movements, alternative HBGA conformations, and HBGA rotations. We showed that a loop (residues 391 to 395) was elegantly repositioned to allow for Lewis Y binding. This loop was also slightly shifted to provide direct hydrogen- and water-mediated bonds with Lewis B. We considered that the flexible loop modulated Lewis HBGA binding. The GII.4 noroviruses have dominated outbreaks over the past decade, which may be explained by their exquisite HBGA binding mechanisms, their fondness for Lewis HBGAs, and their temporal amino acid modifications.ImportanceOur data provide a comprehensive picture of GII.4 P domain and HBGA binding interactions. The exceptionally high resolutions of our X-ray crystal structures allowed us to accurately recognize novel GII.4 P domain interactions with numerous HBGA types. We showed that the GII.4 P domain-HBGA interactions involved complex binding mechanisms that were not previously observed in norovirus structural studies. Many of the GII.4 P domain-HBGA interactions we identified were negative in earlier enzyme-linked immunosorbent assay (ELISA)-based studies. Altogether, our data show that the GII.4 norovirus P domains can accommodate numerous HBGA types.

Dataset Information

Low-Frequency Blood Group Antigens in Switzerland.

Publications

Low-Frequency Blood Group Antigens in Switzerland.

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