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Bartonella rochalimae Detection by a Sensitive and Specific PCR Platform.


ABSTRACT: Bartonella rochalimae is an emerging zoonotic pathogen present in the United States, South America, and Europe. The molecular detection of B. rochalimae frequently relies on polymerase chain reaction (PCR) assays that target the genus Bartonella coupled with DNA sequencing for species determination. However, the presence of other Bartonella spp. in the sample being tested may result in false-negative results for B. rochalimae, especially when Sanger sequencing is used. We developed a sensitive and specific quantitative PCR platform for B. rochalimae by targeting the intergenic transcribed spacer, gltA, and rpoB genes, which are recommended for subtyping characterization. This PCR platform achieved the limit of detection between five and 10 genomic equivalents per reaction and did not amplify DNA from other Bartonella species or selected hosts. This PCR platform is a fast and cost-effective option to be used in epidemiological evaluations of reservoirs and vectors and in detecting and quantifying B. rochalimae infection in humans.

SUBMITTER: Chan D 

PROVIDER: S-EPMC6159559 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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<i>Bartonella rochalimae</i> Detection by a Sensitive and Specific PCR Platform.

Chan Dennis D   Geiger Joseph Andrew JA   Vasconcelos Elton José Rosas EJR   Oakley Brian B   Diniz Pedro Paulo Vissotto de Paiva PPVP  

The American journal of tropical medicine and hygiene 20181001 4


<i>Bartonella rochalimae</i> is an emerging zoonotic pathogen present in the United States, South America, and Europe. The molecular detection of <i>B. rochalimae</i> frequently relies on polymerase chain reaction (PCR) assays that target the genus <i>Bartonella</i> coupled with DNA sequencing for species determination. However, the presence of other <i>Bartonella</i> spp. in the sample being tested may result in false-negative results for <i>B. rochalimae</i>, especially when Sanger sequencing  ...[more]

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