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Lateral Flow Test for Visual Detection of Multiple MicroRNAs.

ABSTRACT: The authors describe a rapid and low-cost approach for multiplex microRNA(miRNA) assay on lateral flow nucleic acid biosensor (LFNAB). The principle of assay is based on sandwich-type nucleic acid hybridization reactions to produce gold nanoparticle (GNP)-attached complexes (ssDNA-microRNA-ssDNA/GNPs), which are captured and visualized on the test zone of LFNAB. By designing three different test zones on LFNAB, simultaneous detection of microRNA-21, microRNA-155 and microRNA-210 was achieved with an adding-measuring model by using GNP as visual tag. The method was challenged by testing the microRNAs in spiked serum samples with satisfied results. In our perception, the test is a particularly valuable tool for clinical application and biomedical diagnosis, particularly in limited resource settings.

SUBMITTER: Zheng W

PROVIDER: S-EPMC6159930 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Lateral Flow Test for Visual Detection of Multiple MicroRNAs.

Zheng Wanli W Yao Li L Teng Jun J Yan Chao C Qin Panzhu P Liu Guodong G Chen Wei W

Sensors and actuators. B, Chemical 20180320

The authors describe a rapid and low-cost approach for multiplex microRNA(miRNA) assay on lateral flow nucleic acid biosensor (LFNAB). The principle of assay is based on sandwich-type nucleic acid hybridization reactions to produce gold nanoparticle (GNP)-attached complexes (ssDNA-microRNA-ssDNA/GNPs), which are captured and visualized on the test zone of LFNAB. By designing three different test zones on LFNAB, simultaneous detection of microRNA-21, microRNA-155 and microRNA-210 was achieved wit ...[more]

PMID: 30270990

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