Ontology highlight
ABSTRACT:
SUBMITTER: Vogt S
PROVIDER: S-EPMC6160918 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Vogt Stefan S Stadlmayr Gerhard G Stadlbauer Katharina K Sádio Flávio F Andorfer Peter P Grillari Johannes J Rüker Florian F Wozniak-Knopp Gordana G
Pharmaceutics 20180827 3
Tetraspan proteins are significantly enriched in the membranes of exosomal vesicles (EVs) and their extracellular domains are attractive targets for engineering towards specific antigen recognition units. To enhance the tolerance of a tetraspanin fold to modification, we achieved significant thermal stabilization of the human CD81 large extracellular loop (hCD81 LEL) via <i>de novo</i> disulfide bonds. The best mutants were shown to exhibit a positive shift in the melting temperature (<i>T</i><s ...[more]