Dataset Information

SDH2 is involved in proper hypha formation and virulence in Candida albicans.

ABSTRACT: AIM:To investigate the role of SDH2 in Candida albicans filamentation and virulence. MATERIALS & METHODS:Caenorhabditis elegans and mouse candidiasis models were used to assess the virulence of a sdh2?/? mutant. Various hypha-inducing media were used to evaluate the hyphal development of C. albicans. DCFH-DA was used to measure intracellular Reactive Oxygen Species (ROS) levels. RESULTS:The sdh2?/? mutant was avirulent in the C. elegans model, hypovirulent in a murine candidiasis model, and defective to form filaments both in vitro and in vivo. Intracellular ROS level increased in the sdh2?/? mutant, and the filamentation defects of sdh2?/? were rescued by decreasing intracellular ROS. CONCLUSION:SDH2 plays an important role in C. albicans filamentation and virulence probably through affecting intracellular ROS. [Formula: see text].

SUBMITTER: Bi S

PROVIDER: S-EPMC6161146 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

SDH2 is involved in proper hypha formation and virulence in Candida albicans.

Bi Shuang S Lv Quan-Zhen QZ Wang Tian-Tian TT Fuchs Beth Burgwyn BB Hu Dan-Dan DD Anastassopoulou Cleo G CG Desalermos Athanasios A Muhammed Maged M Wu Chin-Lee CL Jiang Yuan-Ying YY Mylonakis Eleftherios E Wang Yan Y

Future microbiology 20180816

<h4>Aim</h4>To investigate the role of SDH2 in Candida albicans filamentation and virulence.<h4>Materials & methods</h4>Caenorhabditis elegans and mouse candidiasis models were used to assess the virulence of a sdh2Δ/Δ mutant. Various hypha-inducing media were used to evaluate the hyphal development of C. albicans. DCFH-DA was used to measure intracellular Reactive Oxygen Species (ROS) levels.<h4>Results</h4>The sdh2Δ/Δ mutant was avirulent in the C. elegans model, hypovirulent in a murine candi ...[more]

PMID: 30113213

Similar Datasets

Project description:UnlabelledCandida albicans is a major life-threatening human fungal pathogen in the immunocompromised host. Host defense against systemic Candida infection relies heavily on the capacity of professional phagocytes of the innate immune system to ingest and destroy fungal cells. A number of pathogens, including C. albicans, have evolved mechanisms that attenuate the efficiency of phagosome-mediated inactivation, promoting their survival and replication within the host. Here we visualize host-pathogen interactions using live-cell imaging and show that viable, but not heat- or UV-killed C. albicans cells profoundly delay phagosome maturation in macrophage cell lines and primary macrophages. The ability of C. albicans to delay phagosome maturation is dependent on cell wall composition and fungal morphology. Loss of cell wall O-mannan is associated with enhanced acquisition of phagosome maturation markers, distinct changes in Rab GTPase acquisition by the maturing phagosome, impaired hyphal growth within macrophage phagosomes, profound changes in macrophage actin dynamics, and ultimately a reduced ability of fungal cells to escape from macrophage phagosomes. The loss of cell wall O-mannan leads to exposure of β-glucan in the inner cell wall, facilitating recognition by Dectin-1, which is associated with enhanced phagosome maturation.ImportanceInnate cells engulf and destroy invading organisms by phagocytosis, which is essential for the elimination of fungal cells to protect against systemic life-threatening infections. Yet comparatively little is known about what controls the maturation of phagosomes following ingestion of fungal cells. We used live-cell microscopy and fluorescent protein reporter macrophages to understand how C. albicans viability, filamentous growth, and cell wall composition affect phagosome maturation and the survival of the pathogen within host macrophages. We have demonstrated that cell wall glycosylation and yeast-hypha morphogenesis are required for disruption of host processes that function to inactivate pathogens, leading to survival and escape of this fungal pathogen from within host phagocytes. The methods employed here are applicable to study interactions of other pathogens with phagocytic cells to dissect how specific microbial features impact different stages of phagosome maturation and the survival of the pathogen or host.

Dataset Information

SDH2 is involved in proper hypha formation and virulence in Candida albicans.

Publications

SDH2 is involved in proper hypha formation and virulence in Candida albicans.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets