Modulation of Ca_V1.3b L-type calcium channels by M₁ muscarinic receptors varies with Ca_Vβ subunit expression.

ABSTRACT:

Objectives

We examined whether two G protein-coupled receptors (GPCRs), muscarinic M₁ receptors (M₁Rs) and dopaminergic D₂ receptors (D₂Rs), utilize endogenously released fatty acid to inhibit L-type Ca²⁺ channels, Ca_V1.3. HEK-293 cells, stably transfected with M₁Rs, were used to transiently transfect D₂Rs and Ca_V1.3b with different Ca_Vβ-subunits, allowing for whole-cell current measurement from a pure channel population.

Results

M₁R activation with Oxotremorine-M inhibited currents from Ca_V1.3b coexpressed with α₂δ-1 and a β_1b, β_2a, β₃, or β₄-subunit. Surprisingly, the magnitude of inhibiti

SUBMITTER: Roberts-Crowley ML 

PROVIDER: S-EPMC6161362 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature