Dataset Information

Suppression of LOX activity enhanced seed vigour and longevity of tobacco (Nicotiana tabacum L.) seeds during storage.

ABSTRACT: The preservation of seed viability and quality in storage is an important trait both for commercial and germplasm usage. To better explore potential mechanisms of tobacco seed deterioration, seed packed in cloth bag (C) and vacuum bag (V) were stored under room temperature (RT) and low temperature (LT, 18°C), and sampled periodically for laboratory testing. Seed stored in low temperature with vacuum bag (LT/V) owned the highest seed vigour after 25 months of storage and in room temperature with cloth bag (RT/C) lost seed vigour and germination ability after 20-month storage. Meanwhile, seed in RT/C notably increased about 5-fold endogenous hydrogen peroxide (H₂O₂), 4-fold malondialdehyde (MDA) contents, 12-fold Lipoxygenases (LOX) activity and 2-fold the expression of NtLOX3 comparing with LT/V at the end of 15-month storage. In addition, regression analysis indicated that LOX activity was strongly negatively correlated with seed vigour as the R ² value reached 0.970 in RT/C. Furthermore, caffeic acid and catechin, the inhibitors of LOX activity, were applied to tobacco seeds pre-treatment and followed with artificial accelerated aging. Seeds pretreated with inhibitors, especially caffeic acid, reduced LOX activity by 50%, MDA and H₂O₂ contents by 40% and 20%, respectively, and increased more than 1.2-fold seed vigour and seedling quality comparing with seeds pretreated with H₂O after 6-day artificial aging, indicating a better seed storability after artificial accelerated aging. These results suggest that LOX accelerated seed aging, and suppression of LOX activity enhanced seed vigour and viability in accelerated aging tobacco seed, opening new opportunities for effective management of seed germplasm under long-term storage and conservation.

SUBMITTER: Li Z

PROVIDER: S-EPMC6161406 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Suppression of LOX activity enhanced seed vigour and longevity of tobacco (<i>Nicotiana tabacum</i> L.) seeds during storage.

Li Zhan Z Gao Yue Y Lin Cheng C Pan Ronghui R Ma Wenguang W Zheng Yunye Y Guan Yajing Y Hu Jin J

Conservation physiology 20180928 1

The preservation of seed viability and quality in storage is an important trait both for commercial and germplasm usage. To better explore potential mechanisms of tobacco seed deterioration, seed packed in cloth bag (C) and vacuum bag (V) were stored under room temperature (RT) and low temperature (LT, 18°C), and sampled periodically for laboratory testing. Seed stored in low temperature with vacuum bag (LT/V) owned the highest seed vigour after 25 months of storage and in room temperature with ...[more]

PMID: 30279990

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Project description:BackgroundPollen tube growth is essential for plant reproduction and represents a widely employed model to investigate polarized cell expansion, a process important for plant morphogenesis and development. Cellular and regulatory mechanisms underlying pollen tube elongation are under intense investigation, which stands to greatly benefit from a comprehensive understanding of global gene expression profiles in pollen and pollen tubes. Here, RNA sequencing technology was applied to de novo assemble a Nicotiana tabacum male gametophytic transcriptome and to compare transcriptome profiles at two different stages of gametophyte development: mature pollen grains (MPG) and pollen tubes grown for six hours in vitro (PT6).ResultsDe novo assembly of data obtained by 454 sequencing of a normalized cDNA library representing tobacco pollen and pollen tube mRNA (pooled mRNA isolated from mature pollen grains [MPG] and from pollen tubes grown in vitro for 3 [PT3] or 6 [PT6] hours) resulted in the identification of 78,364 unigenes. Among these unigenes, which mapped to 24,933 entries in the Sol Genomics Network (SGN) N. tabacum unigene database, 24,672 were predicted to represent full length cDNAs. In addition, quantitative analyses of data obtained by Illumina sequencing of two separate non-normalized MPG and PT6 cDNA libraries showed that 8979 unigenes were differentially expressed (differentially expressed unigenes: DEGs) between these two developmental stages at a FDR q-value of <0.0001. Interestingly, whereas most of these DEGs were downregulated in PT6, the minor fraction of DEGs upregulated in PT6 was enriched for GO (gene ontology) functions in pollen tube growth or fertilization.ConclusionsA major output of our study is the development of two different high-quality databases representing the tobacco male gametophytic transcriptome and containing encompassing information about global changes in gene expression after pollen germination. Quantitative analyses of these databases 1) indicated that roughly 30% of all tobacco genes are expressed in the male gametophyte, and 2) support previous observations suggesting a global reduction of transcription after pollen germination. Interestingly, a small number of genes, many of which predicted to function in pollen tube growth or fertilization, were found to be upregulated in elongating pollen tubes despite globally reduced transcription.

Dataset Information

Suppression of LOX activity enhanced seed vigour and longevity of tobacco (Nicotiana tabacum L.) seeds during storage.

Publications

Suppression of LOX activity enhanced seed vigour and longevity of tobacco (<i>Nicotiana tabacum</i> L.) seeds during storage.

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