Dataset Information

Genetic characterization of the artisanal mud crab fishery in Myanmar.

ABSTRACT: Fish are important for food supply, especially in developing countries. In Southeast Asia, including Myanmar, the mud crab fishery is an important livelihood that represents a valuable source of income and food to coastal communities. However, the increasing demand for mud crab in domestic and international markets and poor management has generated concern about the status of this fishery across Southeast Asia. In this region, at least four species of mud crab in the genus Scylla are recognised but their correct identification and occurrence remain to be fully explained. Relying on accurate taxonomic identification of mud crab species represents the cornerstone of the successful implementation of management plans as life history biology and relative exploitation rates may vary by species due to gear susceptibility. Toward this aim, tissue samples from mud crabs were collected from four fishing communities of the Mergui archipelago, in the Tanintharyi region of southern Myanmar. All crab samples were DNA barcoded for species identification through sequencing. This study is the first genetic characterization of the mud crab fishery in Myanmar and revealed that Scylla olivacea was the only species found in the sampled fisheries of the Tanintharyi region. The populations studied across the Mergui archipelago did not show evidence of genetic structure, but gene flow appeared to be limited among conspecifics from neighbouring countries.

SUBMITTER: Segura-Garcia I

PROVIDER: S-EPMC6161904 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Genetic characterization of the artisanal mud crab fishery in Myanmar.

Segura-García Iris I Tun Thu Yain TY Box Stephen J SJ

PloS one 20180928 9

Fish are important for food supply, especially in developing countries. In Southeast Asia, including Myanmar, the mud crab fishery is an important livelihood that represents a valuable source of income and food to coastal communities. However, the increasing demand for mud crab in domestic and international markets and poor management has generated concern about the status of this fishery across Southeast Asia. In this region, at least four species of mud crab in the genus Scylla are recognised ...[more]

PMID: 30265718

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Project description:BackgroundInfection of bacterial Vibrio parahaemolyticus is common in mud crab farms. However, the mechanisms of the crab's response to pathogenic V. parahaemolyticus infection are not fully understood. MicroRNAs (miRNAs) are a class of small noncoding RNAs that function as regulators of gene expression and play essential roles in various biological processes. To understand the underlying mechanisms of the molecular immune response of the crab to the pathogens, high-throughput Illumina/Solexa deep sequencing technology was used to investigate the expression profiles of miRNAs in S. paramamosain under V. parahaemolyticus infection.Methodology/principal findingsTwo mixed RNA pools of 7 tissues (intestine, heart, liver, gill, brain, muscle and blood) were obtained from V. parahaemolyticus infected crabs and the control groups, respectively. By aligning the sequencing data with known miRNAs, we characterized 421 miRNA families, and 133 conserved miRNA families in mud crab S. paramamosain were either identical or very similar to existing miRNAs in miRBase. Stem-loop qRT-PCRs were used to scan the expression levels of four randomly chosen differentially expressed miRNAs and tissue distribution. Eight novel potential miRNAs were confirmed by qRT-PCR analysis and the precursors of these novel miRNAs were verified by PCR amplification, cloning and sequencing in S. paramamosain. 161 miRNAs (106 of which up-regulated and 55 down-regulated) were significantly differentially expressed during the challenge and the potential targets of these differentially expressed miRNAs were predicted. Furthermore, we demonstrated evolutionary conservation of mud crab miRNAs in the animal evolution process.Conclusions/significanceIn this study, a large number of miRNAs were identified in S. paramamosain when challenged with V. parahaemolyticus, some of which were differentially expressed. The results show that miRNAs might play some important roles in regulating gene expression in mud crab under V. parahaemolyticus infection, providing a basis for further investigation of miRNA-modulating networks in innate immunity of mud crab.

Dataset Information

Genetic characterization of the artisanal mud crab fishery in Myanmar.

Publications

Genetic characterization of the artisanal mud crab fishery in Myanmar.

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