Dataset Information

A modified serine cycle in Escherichia coli coverts methanol and CO₂ to two-carbon compounds.

ABSTRACT: Microbial utilization of renewable one-carbon compounds, such as methane, methanol, formic acid, and CO₂, has emerged as a potential approach to increase the range of carbon sources for bioproduction and address climate change issues. Here, we modify the natural serine cycle present in methylotrophs and build an adapted pathway for Escherichia coli, which allows microorganism to condense methanol (or formate) together with bicarbonate to produce various products. We introduce the modified cycle into E. coli and demonstrate its capability for one-carbon assimilation through growth complementation and isotope labeling experiments. We also demonstrate conversion of methanol to ethanol by utilizing the modified serine cycle in an engineered E. coli strain, achieving a reaction yet to be accomplished by a one-pot chemical process. This work provides a platform to utilize various renewable one-carbon compounds as carbon sources for biosynthesis through a modified serine cycle in E. coli.

SUBMITTER: Yu H

PROVIDER: S-EPMC6162302 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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A modified serine cycle in Escherichia coli coverts methanol and CO<sub>2</sub> to two-carbon compounds.

Yu Hong H Liao James C JC

Nature communications 20180928 1

Microbial utilization of renewable one-carbon compounds, such as methane, methanol, formic acid, and CO<sub>2</sub>, has emerged as a potential approach to increase the range of carbon sources for bioproduction and address climate change issues. Here, we modify the natural serine cycle present in methylotrophs and build an adapted pathway for Escherichia coli, which allows microorganism to condense methanol (or formate) together with bicarbonate to produce various products. We introduce the modi ...[more]

PMID: 30266898

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Project description:The increasing demand for non-food competitive carbon sources such as methanol for biotechnology has brought methanol-utilizing bacteria, so-called methylotrophs, to focus. The product spectrum of natural methylotrophs and their genetic accessibility is limited and as an alternative approach, the introduction of methylotrophic metabolism into a biotechnologically well-established organism, such as Escherichia coli, represents a promising concept. By performing long-term evolution over 600 days, we obtained an E. coli strain that is able to grow on methanol as its sole carbon source at rates comparable to natural methylotrophic organisms. We confirmed that the strain forms its entire biomass from methanol. Furthermore, we sequenced the genome of the evolved strain and compared it to the genome of its ancestor. Intriguingly, we found several hundreds of mutations targeting genes of various functions, such as catalysis and regulation. Like the comparison of the genome before and after evolution, the investigation of the proteome would be of high interest. Proteomics would reveal the consequences of the regulatory mutations found in the genome and provide an overall picture of the adaptations by the cell enabling it to grow on methanol. The increasing demand for non-food competitive carbon sources such as methanol for biotechnology has brought methanol-utilizing bacteria, so-called methylotrophs, to focus. The product spectrum of natural methylotrophs and their genetic accessibility is limited and as an alternative approach, the introduction of methylotrophic metabolism into a biotechnologically well-established organism, such as Escherichia coli, represents a promising concept. By performing long-term evolution over 600 days, we obtained an E. coli strain that is able to grow on methanol as its sole carbon source at rates comparable to natural methylotrophic organisms. We confirmed that the strain forms its entire biomass from methanol. Furthermore, we sequenced the genome of the evolved strain and compared it to the genome of its ancestor. Intriguingly, we found several hundreds of mutations targeting genes of various functions, such as catalysis and regulation. Like the comparison of the genome before and after evolution, the investigation of the proteome would be of high interest. Proteomics would reveal the consequences of the regulatory mutations found in the genome and provide an overall picture of the adaptations by the cell enabling it to grow on methanol.

Dataset Information

A modified serine cycle in Escherichia coli coverts methanol and CO₂ to two-carbon compounds.

Publications

A modified serine cycle in Escherichia coli coverts methanol and CO<sub>2</sub> to two-carbon compounds.

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Dataset Information

A modified serine cycle in Escherichia coli coverts methanol and CO2 to two-carbon compounds.

Publications

A modified serine cycle in Escherichia coli coverts methanol and CO<sub>2</sub> to two-carbon compounds.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

A modified serine cycle in Escherichia coli coverts methanol and CO₂ to two-carbon compounds.