Ontology highlight
ABSTRACT:
SUBMITTER: Zhang BS
PROVIDER: S-EPMC6163054 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Chembiochem : a European journal of chemical biology 20180131 5
New applications for bioluminescence imaging require an expanded set of luciferase enzymes and luciferin substrates. Here, we report two novel luciferins for use in vitro and in cells. These molecules comprise regioisomeric pyridone cores that can be accessed from a common synthetic route. The analogues exhibited unique emission spectra with firefly luciferase, although photon intensities remained weak. Enhanced light outputs were achieved by using mutant luciferase enzymes. One of the luciferin ...[more]