Dataset Information

The MADS-box Transcription Factor PsMAD1 Is Involved in Zoosporogenesis and Pathogenesis of Phytophthora sojae.

ABSTRACT: Transcriptional regulation is critical for plant pathogen development and virulence. MADS-box transcription factors belong to a highly conserved transcriptional regulator family in eukaryotic organisms that are involved in various important biological processes. Only one predicted MADS-box gene, PsMAD1, was identified in Phytophthora sojae, which was highly expressed during the sporangia and infection stages. To investigate its function, we generated PsMAD1 knockout mutants using the CRISPR/Cas9 system. Compared with the wild-type strain, the mutants showed no changes in vegetative growth, oospore production, or no differences in sensitivity to various abiotic stresses. Although sporangia production was normal, no zoospore release was detected in PsMAD1 mutants. Microscopy analyses revealed failure of cleavage of the cytoplasm into uninucleate zoospores in the mutants. In addition, the mutants showed reduced virulence in soybean. RNA-seq data indicated that PsMAD1 may regulate many zoospore development and infection associated genes. Thus, PsMAD1 may be a major regulator of P. sojae involved in zoosporogenesis and pathogenesis.

SUBMITTER: Lin L

PROVIDER: S-EPMC6165875 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

The MADS-box Transcription Factor PsMAD1 Is Involved in Zoosporogenesis and Pathogenesis of Phytophthora sojae.

Lin Long L Ye Wenwu W Wu Jiawei J Xuan Mingrun M Li Yufei Y Gao Jian J Wang Yonglin Y Wang Yan Y Dong Suomeng S Wang Yuanchao Y

Frontiers in microbiology 20180924

Transcriptional regulation is critical for plant pathogen development and virulence. MADS-box transcription factors belong to a highly conserved transcriptional regulator family in eukaryotic organisms that are involved in various important biological processes. Only one predicted MADS-box gene, PsMAD1, was identified in Phytophthora sojae, which was highly expressed during the sporangia and infection stages. To investigate its function, we generated PsMAD1 knockout mutants ...[more]

PMID: 30319576

Similar Datasets

Project description:BackgroundBasic leucine zipper (bZIP) transcription factors are present exclusively in eukaryotes and constitute one of the largest and most diverse transcription factor families. The proteins are responsible for central developmental and physiological processes in plants, animals, and fungi, including the pathogenicity of fungal plant pathogens. However, there is limited understanding of bZIPs in oomycetes, which are fungus-like organisms in the kingdom Stramenopila. Oomycetes include many destructive plant pathogens, including the well-studied species Phytophthora sojae, which causes soybean stem and root rot.ResultsCandidate bZIPs encoded in the genomes of P. sojae and four other oomycetes, two diatoms, and two fungal species were predicted using bioinformatic methods. Comparative analysis revealed expanded numbers of bZIP candidates in oomycetes, especially the Phytophthora species, due to the expansion of several novel bZIP classes whose highly conserved asparagines in basic DNA-binding regions were substituted by other residues such as cysteine. The majority of these novel bZIP classes were mostly restricted to oomycetes. The large number of novel bZIPs appears to be the result of widespread gene duplications during oomycete evolution. The majority of P. sojae bZIP candidates, including both conventional and novel bZIP classes, were predicted to contain canonical protein secondary structures. Detection of gene transcripts using digital gene expression profiling and qRT-PCR suggested that most of the candidates were not pseudogenes. The major transcriptional shifts of bZIPs occurred during the zoosporangia/zoospore/cyst and host infection stages. Several infection-associated bZIP genes were identified that were positively regulated by H2O2 exposure.ConclusionsThe identification of large classes of bZIP proteins in oomycetes with novel bZIP motif variants, that are conserved and developmentally regulated and thus presumably functional, extends our knowledge of this important family of eukaryotic transcription factors. It also lays the foundation for detailed studies of the roles of these proteins in development and infection in P. sojae and other oomycetes.

Dataset Information

The MADS-box Transcription Factor PsMAD1 Is Involved in Zoosporogenesis and Pathogenesis of Phytophthora sojae.

Publications

The MADS-box Transcription Factor PsMAD1 Is Involved in Zoosporogenesis and Pathogenesis of <i>Phytophthora sojae</i>.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets