KChIP3 coupled to Ca²⁺ oscillations exerts a tonic brake on baseline mucin release in the colon.

ABSTRACT: Regulated mucin secretion from specialized goblet cells by exogenous agonist-dependent (stimulated) and -independent (baseline) manner is essential for the function of the epithelial lining. Over extended periods, baseline release of mucin can exceed quantities released by stimulated secretion, yet its regulation remains poorly characterized. We have discovered that ryanodine receptor-dependent intracellular Ca²⁺ oscillations effect the dissociation of the Ca²⁺-binding protein, KChIP3, encoded by KCNIP3 gene, from mature mucin-filled secretory granules, allowing for their exocytosis. Increased Ca²⁺ oscillations, or depleting KChIP3, lead to mucin hypersecretion in a human differentiated colonic cell line, an effect reproduced in the colon of Kcnip

SUBMITTER: Cantero-Recasens G 

PROVIDER: S-EPMC6167051 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature