Dataset Information

The Transcriptional Factor PPAR?b Positively Regulates Elovl5 Elongase in Golden Pompano Trachinotus ovatus (Linnaeus 1758).

ABSTRACT: The nuclear peroxisome proliferator-activated receptors (PPARs) regulate the transcription of elongases of very long-chain fatty acids (Elovl), which are involved in polyunsaturated fatty acid (PUFA) biosynthesis in mammals. In the present study, we first characterized the function of Elovl5 elongase in Trachinotus ovatus. The functional study showed that ToElovl5 displayed high elongation activity toward C18 and C20 PUFA. To investigate whether PPAR?b was a regulator of Elovl5, we also reported the sequence of T. ovatus PPAR?b (ToPPAR?b). The open reading frame (ORF) sequence encoded 469 amino acids possessing four typical characteristic domains, including an N-terminal hypervariable region, a DNA-binding domain (DBD), a flexible hinge domain and a ligand-binding domain (LBD). Thirdly, promoter activity experiments showed that the region from PGL3-basic-Elovl5-5 (-146 bp to +459 bp) was defined as the core promoter by progressive deletion mutation of Elovl5. Moreover, PPAR?b overexpression led to a clear time-dependent enhancement of ToElovl5 promoter expression in HEK 293T cells. Fourth, the agonist of PPAR?b prominently increased PPAR?b and Elovl5 expression, while PPAR?b depletion by RNAi or an inhibitor was correlated with a significant reduction of Elovl5 transcription in T. ovatus caudal fin cells (TOCF). In conclusion, the present study provides the first evidence of the positive regulation of Elovl5 transcription by PPAR?b and contributes to a better understanding of the transcriptional mechanism of PPAR?b in fish.

SUBMITTER: Zhu KC

PROVIDER: S-EPMC6167968 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Publications

The Transcriptional Factor PPARαb Positively Regulates Elovl5 Elongase in Golden Pompano Trachinotus ovatus (Linnaeus 1758).

Zhu Ke-Cheng KC Song Ling L Zhao Chao-Ping CP Guo Hua-Yang HY Zhang Nan N Guo Liang L Liu Bao-Suo BS Jiang Shi-Gui SG Zhang Dian-Chang DC

Frontiers in physiology 20180925

The nuclear peroxisome proliferator-activated receptors (PPARs) regulate the transcription of elongases of very long-chain fatty acids (Elovl), which are involved in polyunsaturated fatty acid (PUFA) biosynthesis in mammals. In the present study, we first characterized the function of Elovl5 elongase in Trachinotus ovatus. The functional study showed that ToElovl5 displayed high elongation activity toward C18 and C20 PUFA. To investigate whether PPARαb was a regulator of ...[more]

PMID: 30319448

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Dataset Information

The Transcriptional Factor PPAR?b Positively Regulates Elovl5 Elongase in Golden Pompano Trachinotus ovatus (Linnaeus 1758).

Publications

The Transcriptional Factor PPARαb Positively Regulates <i>Elovl5</i> Elongase in Golden Pompano <i>Trachinotus ovatus</i> (Linnaeus 1758).

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