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Influence of ylHog1 MAPK kinase on Yarrowia lipolytica stress response and erythritol production.


ABSTRACT: Erythritol production is a unique response to hyperosmotic stress that is observed in a small group of yeasts, including Yarrowia lipolytica. This study investigated whether this unusual mechanism is regulated by the HOG pathway, well described in Saccharomyces cerevisiae. The gene YALI0E25135g was identified as the Y. lipolytica homologue of HOG1 and was found to be phosphorylated in response to hyperosmotic shock. Deletion of the gene caused a significant decrease in resistance to hyperosmotic stress and negatively affected erythritol production. Interestingly, the deletion strain yl-hog1? displayed significant morphological defects, with the cells growing in a filamentous form. Moreover, yl-hog1? cells were also resistant to the cell wall damaging agents Congo red and calcofluor white. Collectively, these results indicate that yl-Hog1 is crucial for the cellular response to hyperosmotic stress, plays a role in the induction of erythritol production, and potentially prevents cross-talk with different MAPK signalling pathways in the cell.

SUBMITTER: Rzechonek DA 

PROVIDER: S-EPMC6170386 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Influence of ylHog1 MAPK kinase on Yarrowia lipolytica stress response and erythritol production.

Rzechonek Dorota A DA   Day Alison M AM   Quinn Janet J   Mirończuk Aleksandra M AM  

Scientific reports 20181003 1


Erythritol production is a unique response to hyperosmotic stress that is observed in a small group of yeasts, including Yarrowia lipolytica. This study investigated whether this unusual mechanism is regulated by the HOG pathway, well described in Saccharomyces cerevisiae. The gene YALI0E25135g was identified as the Y. lipolytica homologue of HOG1 and was found to be phosphorylated in response to hyperosmotic shock. Deletion of the gene caused a significant decrease in resistance to hyperosmotic  ...[more]

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