Dataset Information

The Wheat MYB Transcription Factor TaMYB31 Is Involved in Drought Stress Responses in Arabidopsis.

ABSTRACT: Drought is one of the major environmental stresses limiting crop growth and production. MYB family transcription factors play crucial roles in response to abiotic stresses. Previous studies found that TaMYB31 is transcriptionally induced by drought stress. However, the biological functions of TaMYB31 in drought stress responses remained unknown. In this study, three TaMYB31 homoeologous genes from hexaploid wheat, designated TaMYB31-A, TaMYB31-B, and TaMYB31-D, were cloned and characterized. Expression analysis showed that TaMYB31 genes have different tissue expression patterns, and TaMYB31-B has relatively high expression levels in most tested tissues. All the three homoeologs were up-regulated by polyethylene glycol (PEG) 6000 and abscisic acid (ABA) treatments. Subcellular localization analyses revealed that TaMYB31 is localized to the nucleus. Ectopic expression of the TaMYB31-B gene in Arabidopsis affected plants growth and enhanced drought tolerance. In addition, seed germination and seedling root growth of TaMYB31-B transgenic plants were more sensitive to exogenous ABA treatment compared to wild type control. RNA-seq analysis indicated that TaMYB31 functions through up-regulation of wax biosynthesis genes and drought-responsive genes. These results provide evidence that TaMYB31 acts as a positive regulator of drought resistance, and justify its potential application in genetic modification of crop drought tolerance.

SUBMITTER: Zhao Y

PROVIDER: S-EPMC6172359 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

The Wheat MYB Transcription Factor TaMYB31 Is Involved in Drought Stress Responses in Arabidopsis.

Zhao Yue Y Cheng Xiyong X Liu Xiaodan X Wu Huifang H Bi Huihui H Xu Haixia H

Frontiers in plant science 20180928

Drought is one of the major environmental stresses limiting crop growth and production. MYB family transcription factors play crucial roles in response to abiotic stresses. Previous studies found that TaMYB31 is transcriptionally induced by drought stress. However, the biological functions of TaMYB31 in drought stress responses remained unknown. In this study, three TaMYB31 homoeologous genes from hexaploid wheat, designated TaMYB31-A, TaMYB31-B, and TaMYB31-D, ...[more]

PMID: 30323824

Similar Datasets

Project description:The NAC transcription factors play important roles in regulating plant growth, development, and senescence, and responding to biotic and abiotic stressors in plants. A novel coding sequence (1,059 bp) was cloned from hexaploid triticale in this study. The putative protein (352 amino acids) encoded by this sequence was over 95% similar to the amino acid sequence of a NAC protein from Aegilops tauschii (XP020161331), and it formed a clade with Ae. tauschii, durum wheat, and barley. The putative protein contained a conserved nature actomyosin (NAM) domain (129 consecutive amino acids) between the 20th and 148th amino acids at the N-terminus and three transcription activation regions at the C-terminus. The novel gene was identified as a triticale NAC gene localized in the nucleus and designated as TwNAC01 (GenBank accession MG736919). The expression levels of TwNAC01 were the highest in roots, followed by leaves and stems when triticale lines were exposed to drought, polyethylene glycol 6,000 (PEG6000), NaCl, cold, methyl jasmonate (MeJA), and abscisic acid (ABA). Transgenic Arabidopsis thaliana overexpressing TwNAC01 had significantly lower leaf water loss rates and longer roots than wild-type (WT) A. thaliana. Virus-induced silencing of the TwNAC01 gene in triticale delayed root development and decreased length of primary root. Under drought stress, leaves of TwNAC01-silenced triticale had higher levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2), but lower relative water content (RWC), net photosynthetic rate, stomatal conductance, intercellular CO2 concentration, and transpiration rate than the leaves of the WT. Gene overexpression and silencing experiments suggested that TwNAC01 improves plant stress tolerance by increasing root length, regulating the water content of plant leaves by reducing MDA and H2O2 content, and adjusting respiration rate. The results suggest that TwNAC01 is a novel NAC transcription factor gene that can be exploited for triticale and cereal improvement.

Project description:BackgroundDrought stress is one of the major abiotic stresses that affects plant growth and productivity. The GAPCp genes play important roles in drought stress tolerance in multiple species. The aim of this experiment was to identify the core cis-regulatory elements that may respond to drought stress in the GAPCp2 and GAPCp3 promoter sequences.ResultsIn this study, the promoters of GAPCp2 and GAPCp3 were cloned. The promoter activities were significantly improved under abiotic stress via regulation of Rluc reporter gene expression, while promoter sequence analysis indicated that these fragments were not almost identical. In transgenic Arabidopsis with the expression of the GUS reporter gene under the control of one of these promoters, the activities of GUS were strong in almost all tissues except the seeds, and the activities were induced after abiotic stress. The yeast one-hybrid system and EMSA demonstrated that TaMYB bound TaGAPCp2P/3P. By analyzing different 5' deletion mutants of these promoters, it was determined that TaGAPCp2P (- 1312~ - 528) and TaGAPCp3P (- 2049~ - 610), including the MYB binding site, contained enhancer elements that increased gene expression levels under drought stress. We used an effector and a reporter to co-transform tobacco and found that TaMYB interacted with the specific MYB binding sites of TaGAPCp2P (- 1197~ - 635) and TaGAPCp3P (- 1456~ - 1144 and - 718~ - 610) in plant cells. Then, the Y1H system and EMSA assay demonstrated that these MYB binding sites in TaGAPCp2P (- 1135 and - 985) and TaGAPCp3P (- 1414 and - 665) were the target cis-elements of TaMYB. The deletion of the specific MYB binding sites in the promoter fragments significantly restrained the drought response, and these results confirmed that these MYB binding sites (AACTAAA/C) play vital roles in improving the transcription levels under drought stress. The results of qRT-PCR in wheat protoplasts transiently overexpressing TaMYB indicated that the expression of TaGAPCp2/3 induced by abiotic stress was upregulated by TaMYB.ConclusionThe MYB binding sites (AACTAAA/C) in TaGAPCp2P/3P were identified as the key cis-elements for responding to drought stress and were bound by the transcription factor TaMYB.

Dataset Information

The Wheat MYB Transcription Factor TaMYB31 Is Involved in Drought Stress Responses in Arabidopsis.

Publications

The Wheat MYB Transcription Factor TaMYB<sup>31</sup> Is Involved in Drought Stress Responses in <i>Arabidopsis</i>.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets