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Single-cell transcriptomic analyses reveal distinct dorsal/ventral pancreatic programs.

ABSTRACT: The pancreas of vertebrates is separately derived from both the dorsal and ventral endodermal domains. However, the difference between these two programs has been unclear. Here, using a pancreatic determination gene, Pdx1, driven GFP transgenic mouse strain, we identified Pdx1-GFP highly expressing cells (Pdx1high) and Pdx1-GFP lowly expressing cells (Pdx1low) in both embryonic dorsal Pdx1-expressing region (DPR) and ventral Pdx1-expressing region (VPR). We analyzed the transcriptomes of single Pdx1low and Pdx1high cells from the DPR and VPR. In the VPR, Pdx1low cells have an intermediate progenitor identity and can generate hepatoblasts, extrahepatobiliary cells, and Pdx1high pancreatic progenitor cells. In the DPR, Pdx1high cells are directly specified as pancreatic progenitors, whereas Pdx1low cells are precocious endocrine cells. Therefore, our study defines distinct road maps for dorsal and ventral pancreatic progenitor specification. The findings provide guidance for optimization of current ?-cell induction protocols by following the in vivo dorsal pancreatic specification program.

SUBMITTER: Li LC

PROVIDER: S-EPMC6172462 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Single-cell transcriptomic analyses reveal distinct dorsal/ventral pancreatic programs.

Li Lin-Chen LC Qiu Wei-Lin WL Zhang Yu-Wei YW Xu Zi-Ran ZR Xiao Yi-Ni YN Hou Caiying C Lamaoqiezhong Yu Peng P Cheng Xin X Xu Cheng-Ran CR

EMBO reports 20180731 10

The pancreas of vertebrates is separately derived from both the dorsal and ventral endodermal domains. However, the difference between these two programs has been unclear. Here, using a pancreatic determination gene, Pdx1, driven GFP transgenic mouse strain, we identified Pdx1-GFP highly expressing cells (Pdx1high) and Pdx1-GFP lowly expressing cells (Pdx1low) in both embryonic dorsal Pdx1-expressing region (DPR) and ventral Pdx1-expressing region (VPR). We analyzed ...[more]

PMID: 30065074

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Single-cell transcriptomic analyses reveal distinct dorsal/ventral pancreatic programs.

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Single-cell transcriptomic analyses reveal distinct dorsal/ventral pancreatic programs.

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